Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge

doi:10.1016/j.jep.2020.113345

	Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge
	Lin, Ze-min 1; Liu, Yu-ting 1,3; Huang, Yue-teng 2; Yang, Xiao-qian 1; Zhu, Feng-hua 1; Tang, Wei 1,3; Zhao, Wei-min 3,4; He, Shi-jun 1,3; Zuo, Jian-ping 1,3
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2021-01-30
卷号	265 页码:12
关键词	Periploca sepium Bunge Pregnane glycosides Anti-nociceptive activity Anti-inflammatory activity Anti-arthritic activity Inflammatory arthritis
ISSN号	0378-8741
DOI	10.1016/j.jep.2020.113345
通讯作者	Zhao, Wei-min(wmzhao@simm.ac.cn) ; He, Shi-jun(heshijun@simm.ac.cn) ; Zuo, Jian-ping(jpzuo@simm.ac.cn)
英文摘要	Ethnopharmacological relevance: Periploca sepium Bunge (P. sepium) is used in traditional Chinese medicine (TCM) for the treatment of autoimmune diseases, particularly rheumatoid arthritis. Periploca sepium periplosides (PePs), isolated from the root bark of P. sepium, characterized as the cardiac glycosides-free pregnane glycosides fraction, is expected to possess therapeutic potential on inflammatory arthritis. Aim of the study: The current study is designed to evaluate the anti-nociceptive, anti-inflammatory and anti-arthritic activities effects of the PePs. Materials and methods: The anti-nociceptive activity of PePs was examined in the writhing test and hot-plate test in mice. The anti-inflammatory activity of PePs was determined by the 2, 4-dinitro-1-fluorobenzene (DNFB)-induced ear edema model and the carrageenan induced paw edema model in mice. The anti-arthritic activity of PePs was investigated by evaluating the joint inflammation and arthritis pathology in rat adjuvant induced arthritis (AIA) and murine collagen induced arthritis (CIA). Phytohaemagglutinin M (PHA-M) -elicited human peripheral blood mononuclear cells (PBMCs) were further applied to assess the suppressive activity of PePs on IFN-gamma and IL-17 production. Results: PePs treatment markedly decreased the acetic acid-induced visceral nociceptive response and increased the hot-plate pain threshold. Further, oral administration of PePs exhibited anti-inflammatory activity by decreasing DNFB-induced ear edema in mice and carrageenan-induced paw edema in rats. Moreover, oral treatment of PePs ameliorated joint swelling and attenuated bone erosion in rodent arthritis, and the therapeutic benefits were partially attributed to the suppression of proinflammatory cytokines such IFN-gamma and IL-17. Moreover, PePs suppressed the proliferation as well as IFN-gamma and IL-17 secretion in PHA-M-elicited human PBMCs in a concentration dependent manner. Conclusions: Taken together, our results justified the traditional use of Periploca sepium Bunge for the treatment of diseases associated with inflammation and pain.
资助项目	National Natural Science Foundation of China (NSFC)[81903882] ; National Natural Science Foundation of China (NSFC)[81871240] ; Personalized Medicines--Molecular Signature-based Drug Discovery and Development, Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020107] ; Personalized Medicines--Molecular Signature-based Drug Discovery and Development, Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020236] ; Personalized Medicines--Molecular Signature-based Drug Discovery and Development, Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020369] ; Shanghai Science and Technology Committee[18401931000]
WOS关键词	RHEUMATOID-ARTHRITIS ; ESSENTIAL OIL ; IN-VITRO ; CELLS ; MICE ; ENCEPHALOMYELITIS ; APOPTOSIS ; DRUGS ; IL-17 ; PAIN
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000583286600055
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296436]
专题	中国科学院上海药物研究所
通讯作者	Zhao, Wei-min; He, Shi-jun; Zuo, Jian-ping
作者单位	1.Chinese Acad Sci, Lab Immunopharmacol, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Dept Nat Prod Chem, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Lin, Ze-min,Liu, Yu-ting,Huang, Yue-teng,et al. Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge[J]. JOURNAL OF ETHNOPHARMACOLOGY,2021,265:12.
APA	Lin, Ze-min.,Liu, Yu-ting.,Huang, Yue-teng.,Yang, Xiao-qian.,Zhu, Feng-hua.,...&Zuo, Jian-ping.(2021).Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge.JOURNAL OF ETHNOPHARMACOLOGY,265,12.
MLA	Lin, Ze-min,et al."Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge".JOURNAL OF ETHNOPHARMACOLOGY 265(2021):12.