Comprehensive evaluation of NAODESHENG by combining UPLC quantitative fingerprint and antioxidant activity

doi:10.1016/j.jpba.2020.113636

	Comprehensive evaluation of NAODESHENG by combining UPLC quantitative fingerprint and antioxidant activity
	Liao, Jun-cheng 2; Wu, Yun-shan 1,3,4; Xu, Fang-fang 1,3,4; Chen, Wei-ying 1,3,4; Zheng, Zuo-liang 3,4; Han, Xiao-dong 3,4; Liu, Bo 1,3,4; Wang, Shu-mei 2; Guo, De-an 2,3,4,5
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2021-01-30
卷号	193 页码:7
关键词	Determination of content NAODESHENG Multi-wavelength fusion fingerprint Anti-oxidation Traditional Chinese Medicine (TCM)
ISSN号	0731-7085
DOI	10.1016/j.jpba.2020.113636
通讯作者	Wu, Yun-shan() ; Liu, Bo(doctliu@263.net) ; Wang, Shu-mei() ; Guo, De-an()
英文摘要	The screening of marker compound is of great significance to the quality control of traditional Chinese medicine (TCM). One approach which combines fingerprint and biological evaluation has developed rapidly. Multi-wavelength fusion fingerprints and antioxidant activity screening are integrated in this study to evaluate the quality of NAODESHENG. Characteristic multiwavelength fusion fingerprints of 14 batches of samples were generated at five different wavelengths and evaluated by quantitative fingerprinting with ultra-performance liquid chromatography (UPLC). In the quantitative fingerprinting method, 21 components in NAODESHENG were qualitatively and quantitatively analyzed by external standard method. The antioxidant activities of these 21 components was determined by pre-column antioxidant activity test. Multivariate statistical methods such as hierarchical clustering analysis and principal component analysis(PCA) was used to reduce the dimensions and variables from a large number of original data to screening marker compound with bioactivity. Based on the above results, it is suggested that 3'-Methoxy Puerarin and 11 other components should be used as the quality marker of NAODESHENG. This study demonstrates the feasibility of multi-wavelength fusion fingerprinting combined with antioxidant activity analysis, which associates quality control with bioactivity, providing a reliable and efficient method for quantitative assessment of TCM quality consistency. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	National Key Research and Development Program of China[2018YFC1707900] ; National Natural Science Foundation of China[81202398] ; National Natural Science Foundation of China[81703379] ; Specific Research Project of State Administration of TCM of China[JDZX2015207] ; Guangdong Provincial Science and Technology Project[2014A020221035] ; Guangdong Provincial Science and Technology Project[2015A020211025] ; Guangdong Provincial Science and Technology Project[20178030314166] ; Guangdong Provincial Science and Technology Project[20188030322012] ; Special Funds for Inheriting and Developing the Traditional Chinese Medicine from TCM Bureau of Guangdong Province[20191137] ; Special Funds for Inheriting and Developing the Traditional Chinese Medicine from TCM Bureau of Guangdong Province[20191142] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2016QJ05] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2016QJ08] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2015MS03] ; Key Project of High-level University Construction of Guangzhou University of Chinese Medicine[XK2018019]
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000600773600001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296094]
专题	中国科学院上海药物研究所
通讯作者	Wu, Yun-shan; Liu, Bo; Wang, Shu-mei; Guo, De-an
作者单位	1.Guangzhou Key Lab Chiral Res Act Components Tradi, Guangzhou 510006, Peoples R China 2.Guangdong Pharmaceut Univ, Guangzhou 510006, Peoples R China 3.Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou 510006, Peoples R China 4.Guangzhou Univ Chinese Med, Guangdong Prov Key Lab Chinese Med Prevent & Trea, Guangzhou 510006, Peoples R China 5.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Liao, Jun-cheng,Wu, Yun-shan,Xu, Fang-fang,et al. Comprehensive evaluation of NAODESHENG by combining UPLC quantitative fingerprint and antioxidant activity[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2021,193:7.
APA	Liao, Jun-cheng.,Wu, Yun-shan.,Xu, Fang-fang.,Chen, Wei-ying.,Zheng, Zuo-liang.,...&Guo, De-an.(2021).Comprehensive evaluation of NAODESHENG by combining UPLC quantitative fingerprint and antioxidant activity.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,193,7.
MLA	Liao, Jun-cheng,et al."Comprehensive evaluation of NAODESHENG by combining UPLC quantitative fingerprint and antioxidant activity".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 193(2021):7.