Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects

doi:10.1016/j.ijbiomac.2020.11.018

	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects
	Zhang, Xue 2,3; Cai, Zhenyu 2; Mao, Haoyu 2; Hu, Pei 4; Li, Xiaojun 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2021
卷号	166 页码:1387-1395
关键词	Coriolus versicolor Polysaccharides Chemical structure NMR analysis Methylation Immunoregulatory effects Beta-glucan
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2020.11.018
通讯作者	Li, Xiaojun(juniors_li@163.com)
英文摘要	Coriolus versicolor is an edible medicinal mushroom in China. Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Cotiolus versicolor by water extraction and ethanol precipitation. Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data. The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded. With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (1 -> 4)-beta-/(1 -> 3)-beta-D-glucopyranosyl group as backbone with branches attached at 0-6 site. Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-alpha mRNA expression level, and phagocytosis on RAW 2647 cells. These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	High-level Talent Introduction Fund of Yangzhou University[137011476] ; High-level Talent Introduction Fund of Yangzhou University[137011974] ; 2018 Distinguished Doctoral Program of Green Yang Golden Phoenix of Yangzhou City ; China Postdoctoral Science Foundation[2020M671636]
WOS关键词	SULFATED POLYSACCHARIDE ; ANTITUMOR-ACTIVITY ; IN-VIVO ; PURIFICATION ; EXTRACT ; ANTIOXIDANT ; MACROPHAGES ; ACTIVATION ; PEPTIDES ; SPORES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000603588400130
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296061]
专题	中国科学院上海药物研究所
通讯作者	Li, Xiaojun
作者单位	1.Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Weste, Yangzhou 225001, Jiangsu, Peoples R China 2.Yangzhou Univ, Med Coll, Inst Translat Med, Yangzhou 225001, Jiangsu, Peoples R China 3.State Adm Tradit Chinese Med, Key Lab Syndrome Differentiat & Treatment Gastr C, Yangzhou 225001, Jiangsu, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xue,Cai, Zhenyu,Mao, Haoyu,et al. Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,166:1387-1395.
APA	Zhang, Xue,Cai, Zhenyu,Mao, Haoyu,Hu, Pei,&Li, Xiaojun.(2021).Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,166,1387-1395.
MLA	Zhang, Xue,et al."Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 166(2021):1387-1395.