A Peptide Binder of E3 Ligase Adaptor SPOP Disrupts Oncogenic SPOP-Protein Interactions in Kidney Cancer Cells | |
Wang, Zhen1,2; Zhang, Hao2,3; Chen, Baoen2; Ouyang, Sisheng2; Zheng, Tong2; Zhou, Ran2,3; Dong, Ze2; Huang, Yue2,4; Zhang, Tao2; Jiang, Hualiang2,3,4,5 | |
刊名 | CHINESE JOURNAL OF CHEMISTRY |
2020-12-31 | |
页码 | 274-280 |
关键词 | SPOP Protein‐ protein interaction Clear cell Renal Cell Carcinomas Crystal structure Peptide inhibitor |
ISSN号 | 1001-604X |
DOI | 10.1002/cjoc.202000462 |
通讯作者 | Luo, Cheng(cluo@simm.ac.cn) ; Yang, Cai-Guang(yangcg@simm.ac.cn) |
英文摘要 | Main observation and conclusion The E3 ligase adaptor SPOP, overexpressed in the nucleus but frequently dislocated into the cytoplasm in all clear cell Renal Cell Carcinomas (ccRCC), serves as a regulatory hub to promote kidney cancer through the ubiquitination and degradation of multiple downstream cancer proteins. Recently, our identification of a selective small-molecule inhibitor of the SPOP-phosphatase and tensin homolog (PTEN) interaction has demonstrated that the oncogenic SPOP-protein interaction would be a druggable target specific to ccRCC therapy. To our knowledge, this is the first time such a small-molecule inhibitor has been developed. Herein, we have identified a peptide binder for the SPOP-MATH domain that disrupts the oncogenic SPOP-protein interactions in kidney cancer cells. Computational design and biophysical characterization yielded peptide Pep38 that binds to the MATH domain of SPOP and competes on PTEN-binding to SPOP in vitro. The X-ray complex structure reveals that the peptide binder features the following combination: one, a mimic of the native peptide binder and two, an additional beta-strand motif in sequence, which could contribute to increased binding affinity. In order to improve cellular permeability, we fused Pep38 with the delivery peptide TAT to prepare peptide TAT38, which inhibits the endogenous substrate binding to SPOP and suppresses the proliferation of the ccRCC cells. Our identification of the peptide inhibitors for SPOP-protein interactions provides further validation that the oncogenic SPOP-signaling pathway in ccRCC could be a druggable target specifically applicable to the therapy of kidney cancers. |
资助项目 | National Natural Science Foundation of China[21725801] ; National Natural Science Foundation of China[81625022] ; National Natural Science Foundation of China[81430084] ; National Natural Science Foundation of China[21807103] ; National Key New Drug Creation and Manufacturing Program, Ministry of Science and Technology[2018ZX09711002] ; Science and Technology Commission of Shanghai Municipality[18YF1428500] ; Science and Technology Commission of Shanghai Municipality[18431907100] ; China Postdoctoral Science Foundation[2018M640434] ; China Postdoctoral Science Foundation[2017M621570] ; China Postdoctoral Science Foundation[2018M632187] |
WOS关键词 | PROSTATE-CANCER ; DOCKING ; DEGRADATION ; MUTATIONS |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | WILEY-V C H VERLAG GMBH |
WOS记录号 | WOS:000603603500001 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/296021] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Luo, Cheng; Yang, Cai-Guang |
作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.UCAS, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Zhejiang, Peoples R China 5.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 6.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Zhen,Zhang, Hao,Chen, Baoen,et al. A Peptide Binder of E3 Ligase Adaptor SPOP Disrupts Oncogenic SPOP-Protein Interactions in Kidney Cancer Cells[J]. CHINESE JOURNAL OF CHEMISTRY,2020:274-280. |
APA | Wang, Zhen.,Zhang, Hao.,Chen, Baoen.,Ouyang, Sisheng.,Zheng, Tong.,...&Yang, Cai-Guang.(2020).A Peptide Binder of E3 Ligase Adaptor SPOP Disrupts Oncogenic SPOP-Protein Interactions in Kidney Cancer Cells.CHINESE JOURNAL OF CHEMISTRY,274-280. |
MLA | Wang, Zhen,et al."A Peptide Binder of E3 Ligase Adaptor SPOP Disrupts Oncogenic SPOP-Protein Interactions in Kidney Cancer Cells".CHINESE JOURNAL OF CHEMISTRY (2020):274-280. |
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