Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine

doi:10.1007/s12250-021-00362-2

	Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine
	Xie, Yuanchao 2; Guo, Xiaozhen 1; Hu, Tianwen 2; Wei, Daibao 2; Ma, Xiuli 1; Wu, Jiaqiang 1; Huang, Bing 1; Shen, Jingshan 2,3
刊名	VIROLOGICA SINICA
	2021-03-22
页码	9
关键词	Porcine epidemic diarrhea virus (PEDV) Nucleoside analog RNA dependent RNA polymerase (RdRp) Antiviral activity
ISSN号	1674-0769
DOI	10.1007/s12250-021-00362-2
通讯作者	Huang, Bing(hbind@163.com) ; Shen, Jingshan(shenjingshan@simm.ac.cn)
英文摘要	Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is widespread in the world. In recent years, the increased virulence of the virus due to viral variations, has caused great economic losses to the pig industry in many countries. It is always worthy to find effective therapeutic methods for PED. As an important class of antivirals, nucleoside drugs which target viral polymerases have been applied in treating human viral infections for half a century. Herein, we evaluated the anti-PEDV potential of three broad-spectrum antiviral nucleoside analogs, remdesivir (RDV), its parent nucleoside (RDV-N) and beta-d-N-4-hydroxycytidine (NHC). Among them, RDV-N was the most active agent in Vero E6 cells with EC50 of 0.31 mu mol/L, and more potent than RDV (EC50 = 0.74 mu mol/L) and NHC (EC50 = 1.17 mu mol/L). The activity of RDV-N was further confirmed using an indirect immuno-fluorescence assay. Moreover, RDV-N exhibited a good safety profile in cells and in mice. The high sequence similarity of the polymerase functional domains of PEDV with other five porcine coronaviruses indicated a broader antiviral spectrum for the three compounds. Generally, RDV-N is a promising broad-spectrum antiviral nucleoside, and it would be worthy to make some structural modifications to increase its oral bioavailability.
资助项目	Shanghai Science and Technology Committee in China[19430750100] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; Shandong Key Provincial Research and Development Program[2019GNC106044] ; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences[CXGC2016B14]
WOS研究方向	Virology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000631308600001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295471]
专题	中国科学院上海药物研究所
通讯作者	Huang, Bing; Shen, Jingshan
作者单位	1.Shandong Acad Agr Sci, Inst Poultry Sci, Jinan 250023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xie, Yuanchao,Guo, Xiaozhen,Hu, Tianwen,et al. Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine[J]. VIROLOGICA SINICA,2021:9.
APA	Xie, Yuanchao.,Guo, Xiaozhen.,Hu, Tianwen.,Wei, Daibao.,Ma, Xiuli.,...&Shen, Jingshan.(2021).Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine.VIROLOGICA SINICA,9.
MLA	Xie, Yuanchao,et al."Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine".VIROLOGICA SINICA (2021):9.