WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro

	WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro
	Tong Xiankun 1; Qiu Hong 2; Zhang Xin 3; Shi Liping 1; Wang Guifeng 1; Ji Feihong 1; Ding Huiyong 1; Tang Wei 1; Ding Kan 2; Zuo Jianping 1
刊名	Acta Pharmacologica Sinica
	2010
卷号	31 期号:5 页码:585
关键词	Dengue virus sulfated polysaccharide infection BHK cells α-D-glucan
ISSN号	1671-4083
英文摘要	Aim: To investigate the mode of action of WSS45, one sulfated derivative of an α-D-glucan from the Gastrodia elata BI, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication. Methods: Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein. Results: WSS45 effectively inhibited DV2 infection in BHK cells with an EC_(50) value of 0.68±0.17 μg/mL, mainly interfered with virus adsorption, in a very early stage of the Virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line. Conclusion: WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/294385]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院上海药物研究所 2.中国科学院 3.Institution Center for Disease Control and Prevention of Guangdong Province
推荐引用方式 GB/T 7714	Tong Xiankun,Qiu Hong,Zhang Xin,et al. WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro[J]. Acta Pharmacologica Sinica,2010,31(5):585.
APA	Tong Xiankun.,Qiu Hong.,Zhang Xin.,Shi Liping.,Wang Guifeng.,...&Zuo Jianping.(2010).WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.Acta Pharmacologica Sinica,31(5),585.
MLA	Tong Xiankun,et al."WSS45, a sulfated α-D-glucan, strongly interferes with Dengue 2 virus infection in vitro".Acta Pharmacologica Sinica 31.5(2010):585.