Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis

doi:10.1016/j.jprot.2020.103900

	Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis
	Gu, Lei 1,2,3; Liu, Xing 2,3; Wang, Yu-Qiu 1; Zhou, Yan-ting 2,3; Zhu, Hong-wen 2,3; Huang, Jin 1; Lan, Le-fu 4,5; Zheng, Jing 1; Yang, Cai-guang 4,5; Zhou, Hu 2,3,5
刊名	JOURNAL OF PROTEOMICS
	2020-08-30
卷号	226 页码:10
关键词	Staphylococcus epidermidis AbfR transcription factor Oxidation-sensing regulator Oxidative stress TMT10-plex labelling Antibiotic resistance regulator
ISSN号	1874-3919
DOI	10.1016/j.jprot.2020.103900
通讯作者	Zheng, Jing(zhengjing@ecust.edu.cn) ; Yang, Cai-guang(yangcg@simm.ac.cn) ; Zhou, Hu(zhouhu@simm.ac.cn)
英文摘要	Staphylococcus epidermidis is a common causative of nosocomial infections associated with indwelling medical devices. To date, the mechanisms of the pathogenicity and drug resistance of S. epidermidis have not been clearly elucidated. AbfR has been previously identified as an oxidation-sensing regulator that regulates bacterial aggregation and biofilm formation by responding to oxidative stress in S. epidermidis; however, the regulatory pathways of AbfR are underexplored. In this study, we investigated the oxidation-sensing regulatory mechanism of AbfR using TMT10-plex labelling quantitative proteomic and untargeted metabolomic approaches. Integrated analysis of two omics datasets indicated that abfR depletion influenced nucleic acid metabolism and activated the DNA mismatch repair pathway. In addition, several energy-related metabolic pathways, including tricarboxylic acid (TCA) cycle, glycolysis, and arginine metabolism, were remarkably impacted by the deletion of abfR. This study revealed the regulatory networks of the transcription factor AbfR from a multi-omics view and demonstrated that AbfR played a broad role in not only mismatch repair but also energy metabolism, enabling S. epidermidis to constantly sense and adapt to environmental stress. Significance: Staphylococcus epidermidis has emerged as a major nosocomial infection causing pathogen. AbfR, a transcription factor of S. epidermidis, plays an important role in oxidative stress, cell aggregation, and biofilm formation; however, the regulatory mechanism of AbfR is unknown. Using proteomic and metabolomic approaches, this study unveils the global regulatory networks of AbfR, and demonstrates that AbfR not only regulates the DNA mismatch repair pathway by an oxidation sensing mechanism but also affects energy metabolism. This study expands the body of knowledge related to regulatory transcription factors in staphylococci and lays a foundation for future research on clinical infections caused by S. epidermidis.
资助项目	Fundamental Research Funds for the Central Universities ; Science and Technology Commission Shanghai Municipality[17XD1404400] ; National Natural Science Foundation of China[81138046] ; Open Project Program of State Key Laboratory of Innovative Natural Medicine and TCM Injections[QFSKL2017005] ; Nova Development Program of the Shanghai Institute of Materia Medica ; [31500110]
WOS关键词	STAPHYLOCOCCUS-AUREUS ; MARR FAMILY ; ARGININE ; BINDING
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000566934000005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291107]
专题	中国科学院上海药物研究所
通讯作者	Zheng, Jing; Yang, Cai-guang; Zhou, Hu
作者单位	1.East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Number 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gu, Lei,Liu, Xing,Wang, Yu-Qiu,et al. Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis[J]. JOURNAL OF PROTEOMICS,2020,226:10.
APA	Gu, Lei.,Liu, Xing.,Wang, Yu-Qiu.,Zhou, Yan-ting.,Zhu, Hong-wen.,...&Zhou, Hu.(2020).Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis.JOURNAL OF PROTEOMICS,226,10.
MLA	Gu, Lei,et al."Revelation of AbfR in regulation of mismatch repair and energy metabolism in S. epidermidis by integrated proteomic and metabolomic analysis".JOURNAL OF PROTEOMICS 226(2020):10.