Genomic and GeneChip expression profiling reveals the inhibitory effects of Amorphophalli Rhizoma in TNBC cells

doi:10.1016/j.jep.2019.02.004

	Genomic and GeneChip expression profiling reveals the inhibitory effects of Amorphophalli Rhizoma in TNBC cells
	Wu, Chunyu 1; Chen, Mingcang 2,3; Zhang, Qiuhua 4; Yu, Linghong 4; Zhu, Jiayan 4; Gao, Xiufei 5
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2019-05-10
卷号	235 页码:206-218
关键词	Gene chip Amorphophalli Rhizoma Triple-negative breast cancer IPA
ISSN号	0378-8741
DOI	10.1016/j.jep.2019.02.004
通讯作者	Gao, Xiufei(gaoxiufei@163.com)
英文摘要	Ethnopharmacological relevance: Amorphophalli Rhizoma has been widely used as an adjuvant treatment for advanced or metastatic breast cancer, pancreatic cancer, hepatoma, and malignant lymphoma, but its molecular mechanism of action for treatment of metastatic triple-negative breast cancer (TNBC) is generally poorly understood. Aim of the study: To investigate genomic changes related to the inhibitory effect of Amorphophalli Rhizoma and to elucidate the molecular mechanism of this inhibition in MDA-MB-231 TNBC cells. Materials and methods: Gene chip analysis was employed to explore genomic changes caused by Amorphophalli Rhizoma in TNBC cells. Potential classical signaling pathways, upstream regulators, functions, regulatory effects and gene interaction networks were analyzed by Ingenuity Pathway Analysis (IPA). Real-time quantitative PCR (RT-qPCR) and RNA interference (RNAi) assays were used to clarify the roles of potential target genes. Results: In total, 536 significantly upregulated and 648 significantly downregulated genes were identified between the group treated with Amorphophalli Rhizoma extract and that treated with vehicle. Many of these differentially expressed genes (DEGs) in TNBC cells are involved in DNA replication, recombination and repair, the cell cycle, and cellular assembly and organization. Attenuation of KNL1, OLFML2A, RTKN2 and SGO1 gene expression by Amorphophalli Rhizoma significantly induced cell cycle arrest and suppressed cell proliferation and migration. Conclusions: The inhibitory effects of Amorphophalli Rhizoma in TNBC cells likely occur through regulation of the spindle checkpoint, chromosomal and centrosomal instability, and cell membrane stability.
资助项目	National Natural Science Foundation of China[81503581] ; National Natural Science Foundation of China[81704075] ; Natural Science Foundation of Zhejiang Province[LY18H270006] ; Natural Science Foundation of Shanghai[17ZR1430700] ; Shanghai Municipal Commission of Health and Family Planning Foundation[20164Y0064]
WOS关键词	BREAST-CANCER CELLS ; KONJAC MANNAN ; PATHWAY ; PROLIFERATION ; APOPTOSIS ; METASTASIS ; INDUCTION ; TARGET ; NUPR1 ; TBX2
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000463126800021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/290040]
专题	中国科学院上海药物研究所
通讯作者	Gao, Xiufei
作者单位	1.Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Breast Surg Integrated Tradit & Western Med, 725 South Wanping Rd, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Zhejiang Univ Tradit Chinese Med, Hangzhou 310053, Zhejiang, Peoples R China 5.Zhejiang Univ Tradit Chinese Med, Affiliated Hosp 1, 54 Youdian Rd, Hangzhou 310006, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Wu, Chunyu,Chen, Mingcang,Zhang, Qiuhua,et al. Genomic and GeneChip expression profiling reveals the inhibitory effects of Amorphophalli Rhizoma in TNBC cells[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,235:206-218.
APA	Wu, Chunyu,Chen, Mingcang,Zhang, Qiuhua,Yu, Linghong,Zhu, Jiayan,&Gao, Xiufei.(2019).Genomic and GeneChip expression profiling reveals the inhibitory effects of Amorphophalli Rhizoma in TNBC cells.JOURNAL OF ETHNOPHARMACOLOGY,235,206-218.
MLA	Wu, Chunyu,et al."Genomic and GeneChip expression profiling reveals the inhibitory effects of Amorphophalli Rhizoma in TNBC cells".JOURNAL OF ETHNOPHARMACOLOGY 235(2019):206-218.