Metabolic characterization of diabetic retinopathy: An H-1-NMR-based metabolomic approach using human aqueous humor

doi:10.1016/j.jpba.2019.06.013

	Metabolic characterization of diabetic retinopathy: An H-1-NMR-based metabolomic approach using human aqueous humor
	Jin, Huiyi 1,2; Zhu, Bijun 1,2; Liu, Xia 3; Jin, Jing 4; Zou, Haidong 1,2,5
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2019-09-10
卷号	174 页码:414-421
关键词	Metabolomics NMR analysis Aqueous humor Diabetic retinopathy Metabolite-metabolite correlation
ISSN号	0731-7085
DOI	10.1016/j.jpba.2019.06.013
通讯作者	Zhu, Bijun(judyzhu52@163.com) ; Zou, Haidong(zouhaidong@sjtu.edu.cn)
英文摘要	Patients with a long duration of diabetes mellitus (DM) usually have accompanied complications such as diabetic retinopathy (DR), which is a leading cause of blindness and visual impairment among working-age persons in developed countries; nevertheless, some patients have no complications. Thus, various studies, including genomic, transcriptomic, and proteomic studies, have been conducted to identify potential biomarkers for predicting DR and to reveal the underlying disease mechanism. Although metabolomics could be a powerful tool for characterizing aqueous eye fluids and revealing the metabolic signatures of common ocular diseases such as DR, studies about its relationship with DR are limited. Moreover, to our knowledge, no previous study has applied a metabolomic approach to investigate the aqueous humor in DR. Therefore, we performed an NMR-based metabolomic study of the aqueous humor of patients with DM and cataract (DM, n = 13), DR and cataract (DR, n = 14), and senile cataract (CON, n = 7) to investigate the metabolic alterations accompanying the development of DR. Principal component analysis, average change analysis, and heatmap analysis revealed that lactate, succinate, 2-hydroxybutyrate, asparagine, dimethylamine, histidine, threonine, and glutamine were the most altered metabolites that potentially play roles in the development and progression of DR. The highly activated alanine, aspartate, and glutamate metabolic pathway was selected using pathway analysis. The phenotypic metabolomic analyses of the aqueous humor indicated an alteration in the metabolic pathways of energy metabolism and amino acids in DR patients which was to some extent suggestive of the pathophysiological process of mitochondrial dysfunction and oxidative stress/endothelial damage. It provides a proof of concept that metabolomic analysis using the aqueous humor of DM patients may be a reliable method to improve the accuracy of predicting the development and progression of DR. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	Chinese National Natural Science Foundation[81670898] ; Chinese National Natural Science Foundation[81400388] ; National Key R&D Program of China[2016YFC0904800] ; Chronic Diseases Prevention and Treatment Project of Shanghai Shen Kang Hospital Development Centre[SHDC12015315] ; Chronic Diseases Prevention and Treatment Project of Shanghai Shen Kang Hospital Development Centre[SHDC2015644] ; Shanghai Outstanding Academic Leader Program[16XD1402300] ; Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support[20172022] ; Project of Shanghai Science and Technology[17411950200] ; Project of Shanghai Science and Technology[17411950202]
WOS关键词	ADMA ; ACID
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000479328000048
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/288853]
专题	中国科学院上海药物研究所
通讯作者	Zhu, Bijun; Zou, Haidong
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Ophthalmol, Shanghai, Peoples R China 2.Shanghai Key Lab Fundus Dis, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, CAS Key Lab Receptor Res, Shanghai, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Ophthalmol, Shanghai, Peoples R China 5.Shanghai Eye Hosp, Shanghai Eye Dis Prevent & Treatment Ctr, Dept Preventat Ophthalmol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Jin, Huiyi,Zhu, Bijun,Liu, Xia,et al. Metabolic characterization of diabetic retinopathy: An H-1-NMR-based metabolomic approach using human aqueous humor[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2019,174:414-421.
APA	Jin, Huiyi,Zhu, Bijun,Liu, Xia,Jin, Jing,&Zou, Haidong.(2019).Metabolic characterization of diabetic retinopathy: An H-1-NMR-based metabolomic approach using human aqueous humor.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,174,414-421.
MLA	Jin, Huiyi,et al."Metabolic characterization of diabetic retinopathy: An H-1-NMR-based metabolomic approach using human aqueous humor".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 174(2019):414-421.