synchrotronradiationbasedfouriertransforminfraredspectromicroscopyforcharacterizationoftheproteinpeptidedistributioninsinglemicrospheres

doi:10.1016/j.apsb.2015.03.008

	synchrotronradiationbasedfouriertransforminfraredspectromicroscopyforcharacterizationoftheproteinpeptidedistributioninsinglemicrospheres
	Wang Manli 1; Lu Xiaolong 2; Yin Xianzhen 2; Tong Yajun 3; Peng Weiwei 3; Wu Li 2; Li Haiyan 2; Yang Yan 4; Gu Jingkai 4; Xiao Tiqiao 3
刊名	actapharmaceuticasinicab
	2015
卷号	5 期号:3 页码:270
关键词	FTIR SPECTROMICROSCOPY FLUORESCENCE MICROSCOPY RELEASE BEHAVIOR DRUG-DELIVERY MICROSPECTROSCOPY NANOPARTICLES PROTEIN MECHANISM CELLS Fourier-transform infrared spectromicroscopy Micerosphere Exenatide PLGA
ISSN号	2211-3835
DOI	10.1016/j.apsb.2015.03.008
英文摘要	The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide (PLGA) microsphere using synchrotron radiation based Fourier-transform infrared speciiomlcroscopy (SR-FTIR). The representative infrared wavenumbers specific for protein/peptide (Exenatide) and excipient (PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands. For quantitative analysis of the distribution within microspheres, Matlab soft are was used to transform the map file into a 3D matrix and the matiix values specific for the drug and excipient were extracted. Comparison of the normalized SR- FM maps of PLGA and Exenatide indicated that PLGA was unit-on-lily distributed, while Exenatide was relatively non-uniformly distributed in the microspheres. In conclusion, SR-FTIR is a rapid, nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia 'Medico, Chinese Academy of 'Medical Sciences. Production and hosting by Elsevier B.V.
资助项目	[National Natural Science Foundation of China]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/287389]
专题	中国科学院上海药物研究所
作者单位	1.安徽中医药大学 2.中国科学院上海药物研究所 3.中国科学院上海应用物理研究所 4.吉林大学
推荐引用方式 GB/T 7714	Wang Manli,Lu Xiaolong,Yin Xianzhen,et al. synchrotronradiationbasedfouriertransforminfraredspectromicroscopyforcharacterizationoftheproteinpeptidedistributioninsinglemicrospheres[J]. actapharmaceuticasinicab,2015,5(3):270.
APA	Wang Manli.,Lu Xiaolong.,Yin Xianzhen.,Tong Yajun.,Peng Weiwei.,...&Zhang Jiwen.(2015).synchrotronradiationbasedfouriertransforminfraredspectromicroscopyforcharacterizationoftheproteinpeptidedistributioninsinglemicrospheres.actapharmaceuticasinicab,5(3),270.
MLA	Wang Manli,et al."synchrotronradiationbasedfouriertransforminfraredspectromicroscopyforcharacterizationoftheproteinpeptidedistributioninsinglemicrospheres".actapharmaceuticasinicab 5.3(2015):270.