elucidationofthefragmentationpathwaysofacomplex37oglycosylflavonolbycidhcdandpqdonanltqorbitrapvelosprohybridmassspectrometer

	elucidationofthefragmentationpathwaysofacomplex37oglycosylflavonolbycidhcdandpqdonanltqorbitrapvelosprohybridmassspectrometer
	Yang Wenzhi; Wu Wanying; Yang Min; Guo Dean
刊名	chinesejournalofnaturalmedicines
	2015
卷号	13 期号:11 页码:867
关键词	LTQ-Orbitrap Fragmentation pathway
ISSN号	2095-6975
英文摘要	The present study was designed to systematically investigate the ESI-MS n behavior of a complex 3, 7- O -glycosyl flavonol, kaempferol 3- O -α-L-2,3-di- O -β-D-(6- E - p -coumaroyl)glucopyranosyl-rhamnopyranosyl-7- O -α-L-rhamnopyranoside (KO) isolated from Epimedium wushanense , and to address the elimination priority among different glycosylation sites and different sugars n experiment of KO was performed on a hybrid LTQ-Orbitrap Velos Pro mass spectrometer in both negative and positive ion modes by three different fragmentation mechanisms (CID, HCD, and PQD). The CID, HCD, and PQD analyses of KO exhibited remarkable discrimination in respect of the scan range, richness, and distribution of product ions through the entire spectra. KO experienced different fragmentation pathways between two ionization modes: the negative mode CID of KO eliminated the glycosyl portions (priority: 7-sugar > 3-substituent and terminal substituents > inner sugar) and produced aglycone product ions at m 284.03 3 2 together with subsequent i,j X 3 0 cleavages were found characteristic for the positive mode CID-MS n . The fragmentation pathways by CID for KO were proposed by analyzing the high accuracy ESI-MS n data. Complementary structural information of KO regarding the aglycone and glycosyl portions was obtained by analyzing the ESI-MS n data in both ionization modes. In conclusion, the LTQ-Orbitrap method facilitates highly reliable qualitative analysis of bioactive flavonoids with three alternative fragmentation modes.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/287114]
专题	中国科学院上海药物研究所
作者单位	中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Yang Wenzhi,Wu Wanying,Yang Min,et al. elucidationofthefragmentationpathwaysofacomplex37oglycosylflavonolbycidhcdandpqdonanltqorbitrapvelosprohybridmassspectrometer[J]. chinesejournalofnaturalmedicines,2015,13(11):867.
APA	Yang Wenzhi,Wu Wanying,Yang Min,&Guo Dean.(2015).elucidationofthefragmentationpathwaysofacomplex37oglycosylflavonolbycidhcdandpqdonanltqorbitrapvelosprohybridmassspectrometer.chinesejournalofnaturalmedicines,13(11),867.
MLA	Yang Wenzhi,et al."elucidationofthefragmentationpathwaysofacomplex37oglycosylflavonolbycidhcdandpqdonanltqorbitrapvelosprohybridmassspectrometer".chinesejournalofnaturalmedicines 13.11(2015):867.