evaluationofendoproteinaselysctrypsinsequentialdigestionusedinproteomicssamplepreparation

doi:10.1016/S1872-2040(17)60998-8

	evaluationofendoproteinaselysctrypsinsequentialdigestionusedinproteomicssamplepreparation
	Li Q(李倩)1; Feng Y(冯钰)2; Tan MJ(谭敏佳)1; Zhai LH(翟琳辉)1
刊名	chinesejournalofanalyticalchemistry
	2017
卷号	45 期号:3 页码:316
关键词	MASS-SPECTROMETRY PHOSPHOPROTEOMIC ANALYSIS BREAST-CANCER TRYPSIN YEAST QUANTIFICATION IDENTIFICATION PROTEINS Trypsin Lys-C Sequential digestion Proteomics Liquid chromatography-tandem mass spectrometry Missed cleavage
ISSN号	0253-3820
DOI	10.1016/S1872-2040(17)60998-8
英文摘要	Endoproteinase lysine- C ( Lys- C)/ trypsin sequential digestion and trypsin digestion were used in 293T cell proteomics sample preparation and the differences of Lys- C/ trypsin sequential digestion and trypsin digestion in proteomics sample preparation were systematically evaluated. It was found that the number of identified peptides and proteins increased significantly and missed cleavage sites, especially K sites decreased dramatically through Lys- C/ trypsin sequential digestion. The average sequence coverage of identified proteins in Lys- C/ trypsin sequential digestion sample was higher than that in trypsin digestion sample. Besides, different amounts of enzymes were tested to select the optimal usage of enzymes in Lys- C/ trypsin sequential digestion. This study provided the references for proteomics sample preparation.
资助项目	[Youth Science and Technology Talents in Shanghai Sail Plan of China] ; [National Natural Science Foundation of China] ; [Shanghai Science and Technology Committee of China] ; [Changchun Institute of Applied Chemistry, Chinese Academy of Sciences]
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/286498]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院上海药物研究所 2.北京华利世科技有限公司
推荐引用方式 GB/T 7714	Li Q,Feng Y,Tan MJ,et al. evaluationofendoproteinaselysctrypsinsequentialdigestionusedinproteomicssamplepreparation[J]. chinesejournalofanalyticalchemistry,2017,45(3):316.
APA	李倩,冯钰,谭敏佳,&翟琳辉.(2017).evaluationofendoproteinaselysctrypsinsequentialdigestionusedinproteomicssamplepreparation.chinesejournalofanalyticalchemistry,45(3),316.
MLA	李倩,et al."evaluationofendoproteinaselysctrypsinsequentialdigestionusedinproteomicssamplepreparation".chinesejournalofanalyticalchemistry 45.3(2017):316.