danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro

	danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro
	Zhou Rong 1; Wang Ling 1; Xu Xing 2; Chen Jing 2; Hu Lihong 2; Chen Lili 2; Shen Xu 1
刊名	actapharmacologicasinica
	2013
卷号	34 期号:8 页码:1061
关键词	1 8-dihydroxyanthraquinone (danthron) traditional Chinese medicine AMPK SREBP1c fatty acid synthetase cholesterol triglyceride glucose consumption
ISSN号	1671-4083
英文摘要	Aim: To discover the active compound on AMP-activated protein kinase (AMPK) activation and investigate the effects of the active compound 1,8-dihydroxyanthraquinone (danthron) from the traditional Chinese medicine rhubarb on AMPK-mediated lipid and glucose metabolism in vitro. Methods: HepG2 and C2C12 cells were used. Cell viability was determined using MTT assay. Real-time PCR was performed to measure the gene expression. Western blotting assay was applied to investigate the protein phosphorylation level. Enzymatic assay kits were used to detect the total cholesterol (TC), triglyceride (TG) and glucose contents. Results: Danthron (0.1, 1, and 10 μmol/L) dose-dependently promoted the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in both HepG2 and C2C12 cells. Meanwhile, danthron treatment significantly reduced the lipid synthesis related sterol regulatory element-binding protein 1c (SREBP1c) and fatty acid synthetase (FAS) gene expressions, and the TC and TG levels. In addition, danthron treatment efficiently increased glucose consumption. The actions of danthron on lipid and glucose metabolism were abolished or reversed by co-treatment with the AMPK inhibitor compound C. Conclusion: Danthron effectively reduces intracellular lipid contents and enhanced glucose consumption in vitro via activation of AMPK signaling pathway.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/284020]
专题	中国科学院上海药物研究所
作者单位	1.华东理工大学 2.中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Zhou Rong,Wang Ling,Xu Xing,et al. danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro[J]. actapharmacologicasinica,2013,34(8):1061.
APA	Zhou Rong.,Wang Ling.,Xu Xing.,Chen Jing.,Hu Lihong.,...&Shen Xu.(2013).danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro.actapharmacologicasinica,34(8),1061.
MLA	Zhou Rong,et al."danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro".actapharmacologicasinica 34.8(2013):1061.