inductionofleukemiacellapoptosisbycheliensisinainvolvesdownregulationofbcl2expression

doi:10.1111/j.1745-7254.2005.00077.x

	inductionofleukemiacellapoptosisbycheliensisinainvolvesdownregulationofbcl2expression
	Zhong Li 1; Li Chaoming 2; Hao Xiaojiang 2; Lou Liguang 2
刊名	actapharmacologicasinica
	2005
卷号	26 期号:5 页码:623
关键词	PHOSPHORYLATED RAP1B CAMP GONIOTHALAMIN Goniothalamus cheliensis cheliensisin A apoptosis HL-60 cells bcl-2 genes cyclic AMP-dependent protein kinases caspases
ISSN号	1671-4083
DOI	10.1111/j.1745-7254.2005.00077.x
英文摘要	Aim: To investigate the apoptosis-inducing effect of cheliensisin A (GC-51), a novel styryl-lactone isolated from Goniothalamus cheliensis, on human promyelocytic leukemia HL-60 cells and the mechanism of action involved. Methods: Apoptotic cell death was determined by morphological examination and DNA agarose gel electrophoresis. The activity of caspase-3 was assessed using Western blotting and the expression of Bcl-2 and Bax genes was analyzed using the reverse transcription-polymerase chain reaction (RT-PCR) method. Results: GC-51 significantly inhibited the proliferation of HL-60 cells with an IC50 of 2.4 +/- 0. 2 mu mol/L and effectively induced apoptosis in HL-60 cells. Exposure of HL-60 cells to 10 mu mol/L GC-51 for 8 h resulted in approximately 53% of the cells undergoing apoptosis. Caspase-3 was activated in GC-51-treated cells, which was manifested by the appearance of the 17 kDa active form of caspase-3 and the cleavage of poly(ADP-ribose) polymerase (PARP). Meanwhile, GC-51 markedly reduced the expression of the anti-apoptotic gene Bcl-2 and increased the expression of the pro-apoptotic gene Bax. The apoptosis-inducing effect of GC-51 was cAMP-dependent protein kinase (PKA) dependent because PKA, but not the protein kinase C, specific inhibitor H-89, blocked the induction of apoptosis by GC-51 in HL-60 cells. Conclusion: The results demonstrate that GC-51 effectively induces apoptosis in HL-60 cells and that this effect is PKA-dependent and involves the downregulation of Bcl-2 expression and the activation of caspase-3.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/283664]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院上海药物研究所 2.中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Zhong Li,Li Chaoming,Hao Xiaojiang,et al. inductionofleukemiacellapoptosisbycheliensisinainvolvesdownregulationofbcl2expression[J]. actapharmacologicasinica,2005,26(5):623.
APA	Zhong Li,Li Chaoming,Hao Xiaojiang,&Lou Liguang.(2005).inductionofleukemiacellapoptosisbycheliensisinainvolvesdownregulationofbcl2expression.actapharmacologicasinica,26(5),623.
MLA	Zhong Li,et al."inductionofleukemiacellapoptosisbycheliensisinainvolvesdownregulationofbcl2expression".actapharmacologicasinica 26.5(2005):623.