Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy

doi:10.1021/jacs.9b11207

	Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy
	Jiao, Jin-Biao 4,6; Wang, Guan-Zhen 1,3; Hu, Xi-Le 6; Zang, Yi 1; Maisonneuve, Stephane 4; Sedgwick, Adam C.2; Sessler, Jonathan L.2; Xie, Juan 4; Li, Jia 1,3,5; He, Xiao-Peng 6
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2020-01-29
卷号	142 期号:4 页码:1925-1932
ISSN号	0002-7863
DOI	10.1021/jacs.9b11207
通讯作者	Sessler, Jonathan L.(Sessler@cm.utexas.edu) ; Xie, Juan(joanne.xie@ens-cachan.fr) ; Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要	As a result of their high specificity for their corresponding biological targets, peptides have shown significant potential in a range of diagnostic and therapeutic applications. However, their widespread use has been limited by their minimal cell permeability and stability in biological milieus. We describe here a hepta-dicyanomethylene-4H-pyran appended beta-cyclodextrin (DCM7-beta-CD) that acts as a delivery enhancing "host" for 1-bromonaphthalene-modified peptides, as demonstrated with peptide probes P1-P4. Interaction between the fluorescent peptides P1-P3 and DCM7-beta-CD results in the hierarchical formation of unique supramolecular architectures, which we term supramolecular-peptide-dots (Spds). Each Spd (Spd-1, Spd-2, and Spd-3) was found to facilitate the intracellular delivery of the constituent fluorescent probes (P1-P3), thus allowing spatiotemporal imaging of an apoptosis biomarker (caspase-3) and mitosis. Spd-4, incorporating the antimicrobial peptide P4, was found to provide an enhanced therapeutic benefit against both Gram-positive and Gram-negative bacteria relative to P4 alone. In addition, a fluorescent Spd-4 was prepared, which revealed greater bacterial cellular uptake compared to the peptide alone (P4-FITC) in E. coli. (ATCC 25922) and S. aureus (ATCC 25923). This latter observation supports the suggestion that the Spd platform reported here has the ability to facilitate the delivery of a therapeutic peptide and provides an easy-to-implement strategy for enhancing the antimicrobial efficacy of known therapeutic peptides. The present findings thus serve to highlight a new and effective supramolecular delivery approach that is potentially generalizable to overcome limitations associated with functional peptides.
资助项目	National Natural Science Foundation of China[21788102] ; National Natural Science Foundation of China[91853201] ; National Natural Science Foundation of China[21722801] ; National Natural Science Foundation of China[81673489] ; National Natural Science Foundation of China[31871414] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; International Cooperation Program of Shanghai Science and Technology Committee[17520750100] ; Fundamental Research Funds for the Central Universities[222201717003] ; COST Action Functional Glyconanomaterials for the Development of Diagnostics and Targeted Therapeutic Probes[CA18132] ; National Institutes of Health[GM RO1 103790] ; Robert A. Welch Foundation[F-0018]
WOS关键词	CELL-PENETRATING PEPTIDES ; BETA-CYCLODEXTRIN ; SUPRAMOLECULAR COMPLEXES ; SELECTIVE DETECTION ; DELIVERY ; PROTEIN ; CHEMISTRY ; APOPTOSIS ; CASPASE-3 ; EMISSION
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000510531900039
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281309]
专题	中国科学院上海药物研究所
通讯作者	Sessler, Jonathan L.; Xie, Juan; Li, Jia; He, Xiao-Peng
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China 2.Univ Texas Austin, Dept Chem, 105 East 24th St A5300, Austin, TX 78712 USA 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Univ Paris Saclay, ENS Paris Saclay, CNRS, PPSM, 61 Av President Wilson, F-94235 Cachan, France 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Druggabil Res Marine Nat Prod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 6.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Jin-Biao,Wang, Guan-Zhen,Hu, Xi-Le,et al. Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(4):1925-1932.
APA	Jiao, Jin-Biao.,Wang, Guan-Zhen.,Hu, Xi-Le.,Zang, Yi.,Maisonneuve, Stephane.,...&Tian, He.(2020).Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(4),1925-1932.
MLA	Jiao, Jin-Biao,et al."Cyclodextrin-Based Peptide Self-Assemblies (Spds) That Enhance Peptide-Based Fluorescence Imaging and Antimicrobial Efficacy".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.4(2020):1925-1932.