Spatial filter and its application in three-dimensional single molecule localization microscopy

doi:10.1088/2050-6120/ab7e0f

	Spatial filter and its application in three-dimensional single molecule localization microscopy
	Fan, Xiaoming 1,2,3; Hendriks, Johnny 1; Comini, Maddalena 1,5; Katranidis, Alexandros 2; Bueldt, Georg 2,4; Gensch, Thomas 1
刊名	METHODS AND APPLICATIONS IN FLUORESCENCE
	2020-04-01
卷号	8 期号:2 页码:12
关键词	three-dimensional imaging fluorescence dSTORM PALM vesicles mitochondria actin filaments
ISSN号	2050-6120
DOI	10.1088/2050-6120/ab7e0f
通讯作者	Fan, Xiaoming(xiaomingfan2015@sina.com) ; Gensch, Thomas(t.gensch@fz-juelich.de)
英文摘要	Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to determine the axial positions of fluorescent molecules and nanoparticles on the nanometer scale by the usage of two spatial filters, which are placed in two otherwise identical emission detection channels. SFSMLM allows axial localization in a range of ca. 1.5 mu m with a localization precision of 15 - 30 nm in axial direction. The technique was utilized for localizing and imaging small cellular structures - e.g. actin filaments, vesicles and mitochondria - in three dimensions.
资助项目	Deutscher Akademischer Austausch Dienst (DAAD)
WOS关键词	OPTICAL RECONSTRUCTION MICROSCOPY ; SUPERRESOLUTION MICROSCOPY ; FLUORESCENCE MICROSCOPY ; DIFFRACTION-LIMIT ; ACTIN ; DYNAMICS ; TRACKING ; REVEALS
WOS研究方向	Chemistry
语种	英语
出版者	IOP PUBLISHING LTD
WOS记录号	WOS:000521503100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280977]
专题	中国科学院上海药物研究所
通讯作者	Fan, Xiaoming; Gensch, Thomas
作者单位	1.Forschungszentrum Julich, Cellular Biophys, Inst Complex Syst ICS 4, Leo Brandt Str, D-52428 Julich, Germany 2.Forschungszentrum Julich, Mol Biophys, Inst Complex Syst ICS 5, Leo Brandt Str, D-52428 Julich, Germany 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Moscow Inst Phys & Technol, Lab Adv Studies Membrane Prot, Dolgoprudnyi, Russia 5.Univ Oxford, Dept Physiol Anatomy& Genet, Oxford, England
推荐引用方式 GB/T 7714	Fan, Xiaoming,Hendriks, Johnny,Comini, Maddalena,et al. Spatial filter and its application in three-dimensional single molecule localization microscopy[J]. METHODS AND APPLICATIONS IN FLUORESCENCE,2020,8(2):12.
APA	Fan, Xiaoming,Hendriks, Johnny,Comini, Maddalena,Katranidis, Alexandros,Bueldt, Georg,&Gensch, Thomas.(2020).Spatial filter and its application in three-dimensional single molecule localization microscopy.METHODS AND APPLICATIONS IN FLUORESCENCE,8(2),12.
MLA	Fan, Xiaoming,et al."Spatial filter and its application in three-dimensional single molecule localization microscopy".METHODS AND APPLICATIONS IN FLUORESCENCE 8.2(2020):12.