Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus

doi:10.1016/j.actbio.2020.10.026

CORC > 金属研究所 > 中国科学院金属研究所

	Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus
	Zhuang, Yifu 1; Ren, Ling 2; Zhang, Shuyuan 2; Wei, Xiang 3; Yang, Ke 2; Dai, Kerong 1
刊名	ACTA BIOMATERIALIA
	2021
卷号	119 页码:472-484
关键词	Antibacterial effect Copper Titanium alloy Implant-associated infection MRSA
ISSN号	1742-7061
DOI	10.1016/j.actbio.2020.10.026
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Dai, Kerong(krdai@163.com)
英文摘要	Implant-associated infection (IAI) induced by methicillin-resistant Staphylococcus aureus (MRSA) is a devastating complication in the orthopedic clinic. Traditional implant materials, such as Ti6Al4V, are vulnerable to microbial infection. In this study, we fabricated a copper (Cu)-containing titanium alloy (Ti6Al4V-Cu) for the prevention and treatment of MRSA-induced IAI. The material characteristics, antibacterial activity, and biocompatibility of Ti6Al4V-Cu were systematically investigated and compared with those of Ti6Al4V. Ti6Al4V-Cu provided stable and continuous Cu2+ release, at a rate of 0.106 mg/cm(2)/d. Its antibacterial performance against MRSA in vitro was confirmed by plate counting analysis, crystal violet staining, and scanning electron microscopic observations. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that Ti6Al4V-Cu suppressed biofilm formation, virulence, and antibioticresistance of MRSA. The in vivo anti-MRSA effect was investigated in a rat IAI model. Implants were contaminated with MRSA solution, implanted into the femur, and left for 6 weeks. Severe IAI developed in the Ti6Al4V group, with increased radiological score (9.6 +/- 1.3) and high histological score (10.1 +/- 1.9). However, no sign of infection was found in the Ti6Al4V-Cu group, as indicated by decreased radiological score (1.3 +/- 0.4) and low histological score (2.3 +/- 0.5). In addition, Ti6Al4V-Cu had favorable biocompatibility both in vitro and in vivo . In summary, Ti6Al4V-Cu is a promising implant material to protect against MRSA-induced IAI. (C) 2020 Published by Elsevier Ltd on behalf of Acta Materialia Inc.
资助项目	National Natural Science Foundation of China[51631009] ; National Natural Science Foundation of China[51931001]
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000602981500004
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/158825]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yang, Ke; Dai, Kerong
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, Shanghai, Peoples R China 2.Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China 3.Suzhou Silvan Med Co Ltd, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhuang, Yifu,Ren, Ling,Zhang, Shuyuan,et al. Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus[J]. ACTA BIOMATERIALIA,2021,119:472-484.
APA	Zhuang, Yifu,Ren, Ling,Zhang, Shuyuan,Wei, Xiang,Yang, Ke,&Dai, Kerong.(2021).Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus.ACTA BIOMATERIALIA,119,472-484.
MLA	Zhuang, Yifu,et al."Antibacterial effect of a copper-containing titanium alloy against implant-associated infection induced by methicillin-resistant Staphylococcus aureus".ACTA BIOMATERIALIA 119(2021):472-484.