Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30

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	Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30
	Liang Chunyan; Ma Ping
刊名	CHINESE JOURNAL OF CANCER RESEARCH
	2008
卷号	20 期号:2 页码:100-104
关键词	PROTEIN FAMILY MESSENGER-RNA INHIBITOR INTERFERENCE SURVIVIN SIRNA EXPRESSION MEMBER DEATH Livin breast cancer RNA interference apoptosis
ISSN号	1000-9604
其他题名	Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30
英文摘要	Objective: To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30. Methods: Chemically synthesized double stranded RNA( dsRNA) targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamine(TM)2000. The transfection efficiency was observed under a fluorescence confocal microscope. Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemical analysis. The effects on apoptosis of ZR-75-30 cells were assessed by FCAS. Results: The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30. The inhibition rate was 53.66% at mRNA level and 58.32% at protein level. After 24h, (8.36 +/- 0.20)% cells transfected with siRNA were induced to apoptosis. Conclusion: Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30. Livin RNAi has a potential value in gene therapy of breast cancer.
语种	英语
CSCD记录号	CSCD:3299649
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/154107]
专题	金属研究所_中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Liang Chunyan,Ma Ping. Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(2):100-104.
APA	Liang Chunyan,&Ma Ping.(2008).Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30.CHINESE JOURNAL OF CANCER RESEARCH,20(2),100-104.
MLA	Liang Chunyan,et al."Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30".CHINESE JOURNAL OF CANCER RESEARCH 20.2(2008):100-104.