Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy

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	Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
	Wang Weiping 2; Chai Ali 1; Shi Yanxia 1; Xie Xuewen 1; Li Baoju 1
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS
	2015
卷号	35 期号:5 页码:1243-1247
关键词	REAL-TIME PCR PLASMODIOPHORA-BRASSICAE CRUCIFEROUS CROPS RAPID DETECTION DISEASE Fourier transform infrared spectroscopy FTIR Chinese cabbage clubroot Plasmodiophora brassicae Model
ISSN号	1000-0593
其他题名	Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
英文摘要	Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm(-1) were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34. 17+12. 24x(1)-9. 81x(2)-6. 05x(3), r=0. 98 (p<0. 05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1. 60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.
语种	英语
CSCD记录号	CSCD:5410956
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/152742]
专题	金属研究所_中国科学院金属研究所
作者单位	1.Chinese Acad Agr Sci, Institute Vegetables & Flowers, Beijing 100081, Peoples R China Institute Vegetables & Flowers 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Weiping,Chai Ali,Shi Yanxia,et al. Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2015,35(5):1243-1247.
APA	Wang Weiping,Chai Ali,Shi Yanxia,Xie Xuewen,&Li Baoju.(2015).Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,35(5),1243-1247.
MLA	Wang Weiping,et al."Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 35.5(2015):1243-1247.