Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface

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	Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface
	Liu, G ; Lao, RJ ; Xu, L ; Xu, Q ; Li, LY ; Zhang, M ; Shen, H ; Mathur, S ; Fan, CH（樊春海） ; Song, SP（宋世平）
刊名	SENSORS
	2011
卷号	11 期号:8 页码:8018
ISSN号	1424-8220
英文摘要	We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.
收录类别	SCI
语种	英语
WOS记录号	WOS:000294253900042
公开日期	2013-09-11
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/12853]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Liu, G,Lao, RJ,Xu, L,et al. Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface[J]. SENSORS,2011,11(8):8018.
APA	Liu, G.,Lao, RJ.,Xu, L.,Xu, Q.,Li, LY.,...&Song, SP.(2011).Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface.SENSORS,11(8),8018.
MLA	Liu, G,et al."Detection of Single-Nucleotide Polymorphism on uidA Gene of Escherichia coli by a Multiplexed Electrochemical DNA Biosensor with Oligonucleotide-Incorporated Nonfouling Surface".SENSORS 11.8(2011):8018.