A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

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	A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I
	Xu, H ; Gao, SL ; Liu, QW ; Pan, D（潘敦） ; Wang, LH(王丽华） ; Ren, SZ ; Ding, M ; Chen, JW ; Liu, G
刊名	SENSORS
	2011
卷号	11 期号:11 页码:10187
ISSN号	1424-8220
英文摘要	We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.
收录类别	SCI
语种	英语
WOS记录号	WOS:000297698600013
公开日期	2013-09-11
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/12846]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Xu, H,Gao, SL,Liu, QW,et al. A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I[J]. SENSORS,2011,11(11):10187.
APA	Xu, H.,Gao, SL.,Liu, QW.,Pan, D.,Wang, LH.,...&Liu, G.(2011).A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I.SENSORS,11(11),10187.
MLA	Xu, H,et al."A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I".SENSORS 11.11(2011):10187.