Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit

CORC > 金属研究所 > 中国科学院金属研究所

	Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
	Zhang Ning; Jiang Jing; Yang Yanli; Wang Zhihe
刊名	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
	2015
卷号	16 期号:10 页码:845-856
关键词	CELL-WALL INVERTASE APOPLASTIC INVERTASE VACUOLAR INVERTASE PLANT DEVELOPMENT CARBOHYDRATE-METABOLISM EXTRACELLULAR INVERTASE SOLANUM-LYCOPERSICON STRUCTURAL INSIGHTS ECTOPIC EXPRESSION CARBON METABOLISM Invertase inhibitor Fruit development Transient transformation system Solanum lycopersicum Overexpression
ISSN号	1673-1581
其他题名	Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit *
英文摘要	In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
资助项目	[National Natural Science Foundation of China] ; [State Key Laboratory of Plant Physiology and Biochemistry Open Project of China]
语种	英语
CSCD记录号	CSCD:5544667
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/142721]
专题	金属研究所_中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Ning,Jiang Jing,Yang Yanli,et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2015,16(10):845-856.
APA	Zhang Ning,Jiang Jing,Yang Yanli,&Wang Zhihe.(2015).Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,16(10),845-856.
MLA	Zhang Ning,et al."Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 16.10(2015):845-856.