Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution

doi:10.1016/j.atmosenv.2019.05.051

CORC > 地球环境研究所 > 中国科学院地球环境研究所 > 粉尘与环境研究室

	Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution
	Chen, L-W Antony 3,4; Zhang, Mi 4; Liu, Ting 1; Fortier, Karey 3; Chow, Judith C.4,5; Alonzo, Fernanda 3; Kolberg, Rachel 2,3; Cao, Junji 5; Lin, Ge 3; Patel, Tanviben Y.3
刊名	ATMOSPHERIC ENVIRONMENT
	2019-09-15
卷号	213 页码:620-628
关键词	Primary biological aerosol particles Automated particle counting Aerosol size distribution DNA stain PM10 PMcoarse
ISSN号	1352-2310
DOI	10.1016/j.atmosenv.2019.05.051
通讯作者	Chen, L-W Antony(antony.chen@unlv.edu)
英文摘要	Despite being recognized as an important part of particulate matter (PM) air pollution and health risk, bioaerosols have not been quantified as extensively as other PM components for establishing PM standards and management strategies. The challenge lies partly in the lack of practical measurement methods. This study evaluated a filter-based, direct-staining fluorescence microscopy (DS-FM) method that may be adapted to routine air quality monitoring for bioaerosol concentration and size distribution. Through testing with bioaerosol standards made of bacterial cells and fungal spores, the method is shown to have precision, accuracy, detection limit, and dynamic range suitable for most ambient environments. DS-FM was demonstrated with PM samples from an arid urban location in Las Vegas, Nevada during the spring allergy season. Detectable bioaerosols ranged from 0.37 to 16 mu m in geometric diameter and averaged 0.27 +/- 0.23 cm(-3) in number concentration with about 2/3 and 1/3 in the fine (<= 2.5 mu m) and coarse (> 2.5 mu m) mode, respectively. The bioaerosol mass, estimated from the size distribution and an assumed density, was mainly in the coarse mode and accounted for 17 +/- 11% of PM10, 20 +/- 13% of PM10-2.5, and 4 +/- 3% of PM2.5 mass. Rain and high wind speeds appeared to elevate bioaerosol levels. Other advantages of DS-FM include low sample consumption and short turnaround times; a large amount of data can be generated by incorporating the measurement into current long-term air quality networks. Suggestions for using the data to inform bioaerosol origins, contributions, and public health impacts are discussed.
资助项目	School of Public Health, University of Nevada, Las Vegas ; Environmental Analysis Facility, Desert Research Institute ; Hubei Province of China[D20184502] ; Clark County School District
WOS关键词	BIOLOGICAL AEROSOL-PARTICLES ; TOTAL AIRBORNE MICROBES ; SIZE DISTRIBUTIONS ; ORGANIC-CARBON ; CLOUD-WATER ; UV-APS ; HEALTH ; MICROSCOPY ; RAIN ; BACTERIA
WOS研究方向	Environmental Sciences & Ecology ; Meteorology & Atmospheric Sciences
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000484870900059
资助机构	School of Public Health, University of Nevada, Las Vegas ; Environmental Analysis Facility, Desert Research Institute ; Hubei Province of China ; Clark County School District
内容类型	期刊论文
源URL	[http://ir.ieecas.cn/handle/361006/13423]
专题	地球环境研究所_粉尘与环境研究室
通讯作者	Chen, L-W Antony
作者单位	1.Hubei Polytech Univ, Sch Environm Sci & Engn, Hubei Key Lab Mine Environm Pollut Control & Reme, Huangshi 435003, Hubei, Peoples R China 2.Clark Cty Dept Air Qual, Las Vegas, NV 89118 USA 3.Univ Nevada, Sch Publ Hlth, Dept Environm & Occupat Hlth, Las Vegas, NV 89154 USA 4.Desert Res Inst, Div Atmospher Sci, Reno, NV 89512 USA 5.Chinese Acad Sci, Inst Earth Environm, KLACP, Xian 710061, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Chen, L-W Antony,Zhang, Mi,Liu, Ting,et al. Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution[J]. ATMOSPHERIC ENVIRONMENT,2019,213:620-628.
APA	Chen, L-W Antony.,Zhang, Mi.,Liu, Ting.,Fortier, Karey.,Chow, Judith C..,...&Watson, John G..(2019).Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution.ATMOSPHERIC ENVIRONMENT,213,620-628.
MLA	Chen, L-W Antony,et al."Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution".ATMOSPHERIC ENVIRONMENT 213(2019):620-628.