The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens
	Zhang, Le ; Zhao, Xu ; Yan, Saihong ; Zha, Jinmiao ; Ma, Xufa
刊名	ENVIRONMENTAL POLLUTION
	2019-07
卷号	250 页码:990-997
关键词	Chinese rare minnow Cypermethrin Host resistance challenge Immunotoxicity
ISSN号	0269-7491
英文摘要	In the present study, to assess the immunotoxicity of cypermethrin (CYP) in fish, Chinese rare minnows (Gobiocypris rarus) were exposed to environmentally relevant concentrations (0.15, 0.5, and 1.5 mu g/L) of CYP for 28 d and subjected to pathogen challenge trials for 2 d. After 28 d of CYP exposure, the levels of Immunoglobulin M (IgM), Alkaline phosphatase (ALP), and C-reactive protein (CRP) were significantly decreased (p < 0.05) after treatment with 1.5 mu g/L CYP. Moreover, an induction of inflammatory cytokine transcripts (tnfa, il-6, il-8, and il-12) was observed (p < 0.05) after treatment with 1.5 mu g/L CYP. After challenge with Pseudomonas fluorescens (P. fluorescens), plasma lysozyme (LYS) activity at 24 and 48 hours post-injection (hpi) was significantly decreased in the 0.5 and 1.5 mu g/L CYP treatment groups (p < 0.05). Moreover, liver Complement component 3 (C3) and CRP contents at 24 hpi were significantly decreased in the 1.5 CYP treatment group (p <0.05), whereas significant decreases in liver C3 and IgM contents were observed at 48 hpi (p < 0.05). Inhibition of expression of Toll-like receptor-nuclear factor kappa B (TLR-NF-kappa B) signaling pathway-related genes was observed in the CYP treatment groups and resulted in significant down-regulation of inflammatory cytokines (il-1 beta and il-12) in the 1.5 mu g/L CYP treatment group at 48 hpi (p <0.05). Interestingly, the mortality in the 0.5 and 1.5 mu g/L CYP treatments was significantly increased at 48 hpi (p < 0.05). These results indicated that environmentally relevant concentrations of CYP suppressed the Chinese rare minnow immune system and reduced immune defense against bacterial infection, thereby causing subsequent mortality. Meanwhile, our results demonstrated that a subsequent host resistance challenge is an effective method for determining the immunotoxicity of chemicals (e.g., CYP). (C) 2019 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43535]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, 18 Shuangqing Rd, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing Key Lab Ind Wastewater Treatment & Reuse, Beijing 100085, Peoples R China 4.South China Inst Environm Sci, Minist Environm Protect Peoples Republ China, Guangzhou 510655, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Le,Zhao, Xu,Yan, Saihong,et al. The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens[J]. ENVIRONMENTAL POLLUTION,2019,250:990-997.
APA	Zhang, Le,Zhao, Xu,Yan, Saihong,Zha, Jinmiao,&Ma, Xufa.(2019).The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens.ENVIRONMENTAL POLLUTION,250,990-997.
MLA	Zhang, Le,et al."The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens".ENVIRONMENTAL POLLUTION 250(2019):990-997.