POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN

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	POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN
	Waseem, H.; Ali, J.; Jamal, A.; Alt, M., I
刊名	APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
	2019
卷号	17 期号:2 页码:3049-3063
关键词	ARGs MGEs qPCR AMR microbiome
ISSN号	1589-1623
英文摘要	The importance of environmental reservoirs as a source from which antimicrobial resistance emerges and subsequently transferred is already established. To evaluate the role of small scale poultry slaughterhouses in resistance dissemination in Pakistan, a comparative genomic analysis of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in the gut microbiome of broiler and household chickens (Gallus gallus domesticus) was performed. An array of 52 qPCR primers targeting the 16S rRNA gene, ARGs and MGEs was used in the experiment to analyse the abundance of selected genes in collected samples. A total of 22 ARGs and 7 MGEs were detected in all samples. The detection frequency of specific gene classes and diversity of genes was found to be higher in broiler as compared to household chicken. Sun was the most abundant ARG with the highest percent relative abundance (2.4%). Total percent relative abundance of ARGs in broiler chicken was found to be significantly (p < 0.05) greater than household chicken fecal samples. A significant linear correlation (R-2 = 0.89) was found between relative abundances of int1 and total ARGs. The clustering and correlation of selective ARGs with MGEs has implied that small scale poultry slaughterhouses can be a potential source for the dissemination of ARGs to other non-resistant environmental and/or clinical bacteria.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43205]
专题	生态环境研究中心_环境纳米材料实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Lab Environm Nanomat, 18 Shuangqing Rd, Beijing 100085, Peoples R China 2.Quaid I Azam Univ, Dept Microbiol, Environm Microbiol Lab, Islamabad 45320, Pakistan 3.Univ Sialkot, Dept Biotechnol, Punjab 51310, Pakistan
推荐引用方式 GB/T 7714	Waseem, H.,Ali, J.,Jamal, A.,et al. POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN[J]. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH,2019,17(2):3049-3063.
APA	Waseem, H.,Ali, J.,Jamal, A.,&Alt, M., I.(2019).POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN.APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH,17(2),3049-3063.
MLA	Waseem, H.,et al."POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN".APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 17.2(2019):3049-3063.