Changes of the denitrifying communities in a multi-stage free water surface constructed wetland

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Changes of the denitrifying communities in a multi-stage free water surface constructed wetland
	Li, Jing ; Wang, Jun-Tao ; Hu, Hang-Wei ; Cai, Zhang-Jie ; Lei, Yin-Ru ; Li, Wei ; Zhang, Man-Yin ; Li, Zong-Ming ; Zhu, Yi-Nuo ; Cui, Li-Juan
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-02-10
卷号	650 页码:1419-1425
关键词	Free water surface constructed wetland Functional genes Abundance Denitrifiers' community composition
ISSN号	0048-9697
英文摘要	Microorganisms play crucial roles in the nitrogen removal processes of wetlands. However, the key functional genes and microbes related to the nitrogen removal remain largely unknown in the free water surface constructed wetland (FWS CW). Here we studied the abundances of denitrifiers by targeting the key functional genes (nirS, nirK and nosZ) and investigated the community compositions of denitrifiers and their correlations with the abiotic variables in a FWS CW. The increase of nosZ/(nirS + nirK) and nirS/nirK ratios in the outlet indicated a shift of denitrifiers' communities which tended to release less nitrous oxide at the genetic potential level. The denitrifiers dominated the bacterial community which also remarkably changed from the inlet to the outlet. PICRUSt analysis revealed that the denitrifiers contributed to 39.1% of the nitrogen metabolism, 38.9% of the amino acid metabolism and 25.6% of the amino acid related enzymes. Four bacterial genera including Hydrogenophaga, Hylemonella, Aquabacterium and Cellvibrio were detected as the putative keystone denitrifiers. The abundance (nirS, nirK and nosZ) and the relative abundance of putative keystone denitrifiers were significantly correlated with total organic carbon, oxidation-reduction potential and C/N ratio, which could be regarded as the determinants for the denitrification process in the free water. (C) 2018 Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42701]
专题	生态环境研究中心_城市与区域生态国家重点实验室
作者单位	1.Chinese Acad Forestry, Inst Wetland Res, Beijing 100091, Peoples R China 2.Beijing Hanshiqiao Natl Wetland Ecosyst Res Stn, Beijing 101399, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 4.Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic 3010, Australia 5.Beijing Key Lab Wetland Ecol Funct & Restorat, Beijing 100091, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Wang, Jun-Tao,Hu, Hang-Wei,et al. Changes of the denitrifying communities in a multi-stage free water surface constructed wetland[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,650:1419-1425.
APA	Li, Jing.,Wang, Jun-Tao.,Hu, Hang-Wei.,Cai, Zhang-Jie.,Lei, Yin-Ru.,...&Cui, Li-Juan.(2019).Changes of the denitrifying communities in a multi-stage free water surface constructed wetland.SCIENCE OF THE TOTAL ENVIRONMENT,650,1419-1425.
MLA	Li, Jing,et al."Changes of the denitrifying communities in a multi-stage free water surface constructed wetland".SCIENCE OF THE TOTAL ENVIRONMENT 650(2019):1419-1425.