Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells
	Luo, Yali 1; Xie, Heidi Qunhui 1; Chen, Yangsheng 1; Xia, Yingjie ; Sha, Rui 1; Liu, Yiyun 1; Ma, Yongchao 1; Xu, Tong 1; Xu, Li 1; Tsim, Karl Wah-Keung
刊名	CHEMICO-BIOLOGICAL INTERACTIONS
	2019-08-01
卷号	308 页码:164-169
关键词	Dioxin Acetylcholinesterase Muscle contraction Aryl hydrocarbon receptor Primary skeletal muscle cell
ISSN号	0009-2797
英文摘要	Emerging data indicate that prenatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) could interfere with myogenic differentiation in vivo. Acetylcholinesterase (EC3.1.1.7; AChE), an enzyme critical for cholinergic neurotransmission, is abundantly expressed in neurons and mature myotubes, and we recently found that muscle AChE expression was suppressed in parallel with the inhibition of myogenic differentiation upon TCDD treatment in mouse C2C12 cells. This TCDD-induced suppression of muscle AChE was proposed to involve an aryl hydrocarbon receptor (AhR)-independent mechanism, but the precise underlying mechanism remains unclear. Considering the widely recognized role of muscular activity in AChE expression and its potential crosstalk with the AhR signaling pathway, we sought to investigate the effect of TCDD on muscle AChE expression in the presence of muscular activity. Therefore, we employed a highly contractile rat primary skeletal muscle culture system in which AChE activity and the expression of genes related to it (AChE T subunit and collagen Q (ColQ)) were increased during the myogenic differentiation process. Although TCDD treatment successfully induced the expression of genes regulated by AhR activation, the treatment exerted no notable effects on myogenic differentiation. Moreover, muscle AChE enzymatic activity and mRNA level remained unchanged following TCDD treatment, and only ColQ mRNA expression was slightly increased after 4-day treatment with TCDD (10(-10) M). The compensatory role of muscle-contraction-related signaling pathways in this newly identified unresponsiveness of muscle AChE to TCDD warrants further investigation.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42530]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Peoples R China 3.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Luo, Yali,Xie, Heidi Qunhui,Chen, Yangsheng,et al. Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2019,308:164-169.
APA	Luo, Yali.,Xie, Heidi Qunhui.,Chen, Yangsheng.,Xia, Yingjie.,Sha, Rui.,...&Zhao, Bin.(2019).Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells.CHEMICO-BIOLOGICAL INTERACTIONS,308,164-169.
MLA	Luo, Yali,et al."Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells".CHEMICO-BIOLOGICAL INTERACTIONS 308(2019):164-169.