Comparative in Vitro and in Vivo Evaluation of the Estrogenic Effect of Hexafluoropropylene Oxide Homologues

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Comparative in Vitro and in Vivo Evaluation of the Estrogenic Effect of Hexafluoropropylene Oxide Homologues
	Xin, Yan ; Ren, Xiao-Min ; Wan, Bin ; Guo, Liang-Hong
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2019-07-16
卷号	53 期号:14 页码:8371-8380
ISSN号	0013-936X
英文摘要	As alternatives to perfluorooctanoic acid (PFOA), hexafluoropropylene oxide (HFPO) homologues, including hexafluoropropylene oxide dimer acid (HFPO-DA), hexafluoropropylene oxide trimer acid (HFPO-TA), and hexafluoropropylene oxide tetramer acid (HFPO-TeA), have been used in the fluoropolymer industry for a long period of time. These compounds have attracted widespread attention in recent years due to their environmental ubiquity and high bioaccumulation capability, as well as their toxicity. In our study, we evaluated the potential estrogenic effects of HFPOs in comparison to PFOA by ligand binding, transcriptional activity, and in vivo assays. Fluorescence ligand binding assays showed that both HFPO-TA and HFPO-TeA exhibited higher binding affinity to estrogen receptor ligand binding domains (ER-LBDs) than PFOA, with 2.5- and 57.5-fold higher affinity to ER alpha-LBD and 2.6- and 41.8-fold higher affinity to ER beta-LBD, respectively, whereas HFPO-DA exhibited weaker binding affinity than PFOA. Unlike PFOA, HFPO-TA and HFPO-TeA exhibited antagonistic activity toward the ERs' signaling pathway, with HFPO-TeA displaying the strongest potency. In silico study revealed that while PFOA binds with ERs in a similar fashion as 17 beta-estradiol, the HFPOs display an antagonistic binding mode. Using a zebrafish model, we further found that exposure to HFPO homologues significantly altered the levels of sex steroid hormones and vitellogenin. In general, both in vivo and in vitro results indicate that HFPO homologues might exert higher estrogenic effects than PFOA.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42525]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.[Xin, Yan 2.Ren, Xiao-Min 3.Wan, Bin 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, 18 Shuangqing Rd, Beijing 100085, Peoples R China 5.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100039, Peoples R China 6.China Jiliang Univ, Inst Environm & Hlth Sci, 258 Xueyuan St, Hangzhou 310018, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Xin, Yan,Ren, Xiao-Min,Wan, Bin,et al. Comparative in Vitro and in Vivo Evaluation of the Estrogenic Effect of Hexafluoropropylene Oxide Homologues[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2019,53(14):8371-8380.
APA	Xin, Yan,Ren, Xiao-Min,Wan, Bin,&Guo, Liang-Hong.(2019).Comparative in Vitro and in Vivo Evaluation of the Estrogenic Effect of Hexafluoropropylene Oxide Homologues.ENVIRONMENTAL SCIENCE & TECHNOLOGY,53(14),8371-8380.
MLA	Xin, Yan,et al."Comparative in Vitro and in Vivo Evaluation of the Estrogenic Effect of Hexafluoropropylene Oxide Homologues".ENVIRONMENTAL SCIENCE & TECHNOLOGY 53.14(2019):8371-8380.