Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry
	Li, Yiling ; Zhang, Mengni 1; Zheng, Chengbin 1; Hu, Ligang 2; Wang, Chao 3; Jiang, Jie 3; He, Bin 3; Jiang, Guibin
刊名	JOURNAL OF CHROMATOGRAPHY A
	2019-12-06
卷号	1607 页码:-
关键词	Native SDS-PAGE Fluorescent staining ICP-MS Silver-associated proteins Microbes
ISSN号	0021-9673
英文摘要	Characterization of silver-associated proteins is important to elucidate underlined mechanisms of silver-containing materials against microbes. Gel electrophoresis based methods are the most popular and basic strategy for the analysis of biomolecules, i.e., proteins and nucleic acids. It solely provides molecular weights of analytes. Extending the method from molecular weight to elemental composition is highly desired when investigating metal-containing molecules. Herein, a gel electrophoresis based method combining native sodium dodecyl sulfate-polyacrylamide gel electrophoresis (native SDS-PAGE), fluorescent staining, and inductively coupled plasma mass spectrometry (ICP-MS) strategy was developed for separation and detection of silver-associated proteins. Two home-made silver-labeled proteins, carbonic anhydrase and ovalbumin, were used for validation of the strategy performance. Silver-associated proteins in Pseudomonas aeruginosa and Staphylococcus aureus treated with silver nanoparticles were further characterized by this method. Some well-known and new proteins were identified to associate to silver in both P. aeruginosa and S. aureus, demonstrating the feasibility of the developed strategy. In conclusion, the current study provides a convenient method for readily identification of silver-associated proteins in biological samples. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42507]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, Chengdu 610064, Sichuan, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China 4.Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Yiling,Zhang, Mengni,Zheng, Chengbin,et al. Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1607:-.
APA	Li, Yiling.,Zhang, Mengni.,Zheng, Chengbin.,Hu, Ligang.,Wang, Chao.,...&Jiang, Guibin.(2019).Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1607,-.
MLA	Li, Yiling,et al."Analysis of silver-associated proteins in pathogen via combination of native SDS-PAGE, fluorescent staining, and inductively coupled plasma mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1607(2019):-.