A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo
	Wang, Yue 1; Gao, Min 1; Liao, Chunyang 2; Yu, Fabiao ; Chen, Lingxin
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2019-12-12
卷号	301 页码:-
关键词	Mercury poisoning Near-infrared fluorescent probe Ratiometric biomaging Superoxide anion Mercury ion
英文摘要	Mercury exposure is associated with severe oxidative stress especially superoxide anion radicals (O-2(center dot-)). Mercury exposure is common in the clinic, but it is difficult to track. Although many researchers have tried to reveal the mechanism of mercury exposure, it is still remain vague that the differences between acute mercury exposure and chronic mercury exposure as well as the fluctuations in O-2(center dot-) during mercury ion (Hg2+) stress. Thus effective tool for O-2(center dot-) and Hg2+ associated-detection is needed urgently. Herein we have developed a stable near-infrared ratiometric fluorescent probe, HCy-SH, for O-2(center dot-) and Hg2+ associated-detection. Probe HCy-SH was designed and synthesized based on a heptamethine cyanine fluorophore and a thiol-responsive group. The probe HCy-SH can be used for mercury poisoning detection in HEK 293 cells and mice models with low detection limits of 65 nM for O-2(center dot-) and 72 nM for Hg2+. Relying on the probe HCy-SH, we found that O-2(center dot-) burst was much severer in acute mercury exposure than chronic mercury exposure, especially in heart, and Hg2+ mainly accumulated in kidney no matter acute mercury exposure or chronic mercury exposure. The experimental results indicated that the probe HCy-SH was a potential candidate for accurate diagnosis and efficacy evaluation of mercury exposure.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42501]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yue,Gao, Min,Liao, Chunyang,et al. A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,301:-.
APA	Wang, Yue,Gao, Min,Liao, Chunyang,Yu, Fabiao,&Chen, Lingxin.(2019).A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,301,-.
MLA	Wang, Yue,et al."A sulfydryl-based near-infrared ratiometic fluorescent probe for assessment of acute/chronic mercury exposure via associated determination of superoxide anion and mercury ion in cells and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 301(2019):-.