Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis

doi:10.1016/j.marenvres.2019.104866

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis
	Li, Xiao-dong 2,3,4; Yan, Tian 1,3,4; Zhang, Qing-chun 1,3,4; Yu, Ren-cheng 1,3,4; Zhou, Ming-jiang 4
刊名	MARINE ENVIRONMENTAL RESEARCH
	2020-05-01
卷号	157 页码:8
关键词	Karenia mikimotoi Brachionus plicatilis Enzymes inhibition Toxicity mechanism
ISSN号	0141-1136
DOI	10.1016/j.marenvres.2019.104866
通讯作者	Li, Xiao-dong(lixiaodong11@163.com) ; Yan, Tian(tianyan@qdio.ac.cn)
英文摘要	Blooms of the dinoflagellate Karenia mikimotoi have cause great financial losses to the marine aquaculture industry. However, the toxicity mechanism of this species is still not fully known. In this study, we evaluated the short-term effects of K. mikimotoi on the rotifer Brachionus plicatilis by micro and sub micro observing and by measuring inhibition of crucial enzymes. Behaviour disorder, mucus production, corona and cilium damage, vesical production, and body shrinkage occurred within 1 h after rotifers were treated with K. mikimotoi at a density of 3 x 10(4) cells/mL. Enzyme activity assays showed that K. mikimotoi at low densities significantly inhibited multiple enzymes within 3 h, and obvious density-effect trends were also observed. For instance, activity of esterase and acetylcholinesterase of rotifers significantly decreased to 94.3/83.3% and 82.8/66.9% of control treatment values in 30 and 1000 cells/mL algal treatment, respectively. Total ATPase and Na+-K+-ATPase activities of rotifers also decreased to 82.3% and 68.6% of control values in 1000 cells/mL treatment. The LDH releasement test and MDA tests showed no significant difference between algae treatment and control. It suggested that K. mikimotoi might not cause significant cytolysis and oxidative damage to rotifers, but may cause mortality by inhibiting the activity of crucial enzymes, which may lead to cell permeability disorder and body shrinkage.
资助项目	Science & Technology Basic Resources Investigation Program of China[2018FY100206] ; National Nature Science Foundation of China[NSFC 41476102] ; National Nature Science Foundation of China[U1406403] ; National Marine Laboratory Key Program[2015ASKJ02] ; Sino-Australian Centre for Healthy Coasts[2016YFE0101500]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000528613500007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167310]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Li, Xiao-dong; Yan, Tian
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 2.Fujian Agr & Forestry Univ, Coll Anim Sci, Inst Oceanol, Key Lab Marine Biotechnol Fujian Prov, Fuzhou 350002, Fujian, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiao-dong,Yan, Tian,Zhang, Qing-chun,et al. Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis[J]. MARINE ENVIRONMENTAL RESEARCH,2020,157:8.
APA	Li, Xiao-dong,Yan, Tian,Zhang, Qing-chun,Yu, Ren-cheng,&Zhou, Ming-jiang.(2020).Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis.MARINE ENVIRONMENTAL RESEARCH,157,8.
MLA	Li, Xiao-dong,et al."Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis".MARINE ENVIRONMENTAL RESEARCH 157(2020):8.