Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers

doi:10.1016/j.aquaculture.2021.736611

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers
	Su, Rina 3,4; Fang, Jun 1; Wang, Hongxia 2,4; Liu, Baozhong 2,4
刊名	AQUACULTURE
	2021-06-30
卷号	539 页码:11
关键词	Meretrix petechialis Vibiro parahaemolyticus Lipid metabolism Mortality Gene expression
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2021.736611
通讯作者	Wang, Hongxia(hxwang@qdio.ac.cn)
英文摘要	Mass summer mortality events in the clam Meretrix petechialis resulting from Vibrio infection have occurred frequently in recent years, and seriously threaten the clam aquaculture industry. Metabolic processes are known to support host defense and survival in stressful conditions by regulating the allocation of metabolic resources. However, changes in lipid metabolism of clams in response to Vibrio infection are largely undescribed. In the present research, we characterized changes in key gene expression and enzyme activities associated with triglyceride-related metabolism, fatty acid de novo synthesis and fatty acid ?-oxidation in M. petechialis during Vibrio infection. By mining dynamic transcriptome data before the increase in mortality, we found Vibrio infection caused significant changes in the metabolic regulations of carbohydrates, lipids, and amino acids. Our data show that levels of triglycerides (TG) decreased and free fatty acids (FFA) increased in the infected clams. Correspondingly, the synthesis activities of TG were significantly reduced relative to TG lipolysis activities after Vibrio infection. Additionally, FFA de novo synthesis was strengthened, especially before massive mortality occurred, but key genes of the fatty acid ?-oxidation pathway showed no significant changes after Vibrio infection. We detected the expression of key genes related to lipid metabolism in resistant and susceptible clams under uninfected and infected conditions and found that differences in the basal expression of seven key genes (ATGL, MDY, SREBP, FAS, CPT-1, ACS, and ECH) could be used as potential biomarkers to predict different Vibrio resistance in clams. Overall, our results provide a starting point for investigating the relationship between energy metabolism changes and the emergence of massive mortality induced by bacterial infection in clams.
资助项目	National Natural Science Foundation of China[31772845] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; China Agriculture Research System[CARS49] ; National Key R & D Program of China[2019YFD0900700] ; National Science Foundation of Shandong Province, China[ZR2020MD089]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000647595900009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170925]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Hongxia
作者单位	1.Zhejiang Mariculture Res Inst, Wenzhou 325005, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Su, Rina,Fang, Jun,Wang, Hongxia,et al. Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers[J]. AQUACULTURE,2021,539:11.
APA	Su, Rina,Fang, Jun,Wang, Hongxia,&Liu, Baozhong.(2021).Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers.AQUACULTURE,539,11.
MLA	Su, Rina,et al."Lipid metabolism changes in clam Meretrix petechialis in response to Vibrio infection and the identification of Vibrio-resistance markers".AQUACULTURE 539(2021):11.