Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium | |
Guo, Chenchen2; Zheng, Rikuan1,3,4,5; Cai, Ruining1,3,4,5; Sun, Chaomin1,3,4,5; Wu, Shimei2 | |
刊名 | MICROORGANISMS |
2021-04-01 | |
卷号 | 9期号:4页码:16 |
关键词 | lipase cold seep cold-active deep sea overexpression |
DOI | 10.3390/microorganisms9040802 |
通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) ; Wu, Shimei(shimeiwu2016@126.com) |
英文摘要 | The deep ocean microbiota has unexplored potential to provide enzymes with unique characteristics. In order to obtain cold-active lipases, bacterial strains isolated from the sediment of the deep-sea cold seep were screened, and a novel strain gcc21 exhibited a high lipase catalytic activity, even at the low temperature of 4 degrees C. The strain gcc21 was identified and proposed to represent a new species of Pseudomonas according to its physiological, biochemical, and genomic characteristics; it was named Pseudomonas marinensis. Two novel encoding genes for cold-active lipases (Lipase 1 and Lipase 2) were identified in the genome of strain gcc21. Genes encoding Lipase 1 and Lipase 2 were respectively cloned and overexpressed in E. coli cells, and corresponding lipases were further purified and characterized. Both Lipase 1 and Lipase 2 showed an optimal catalytic temperature at 4 degrees C, which is much lower than those of most reported cold-active lipases, but the activity and stability of Lipase 2 were much higher than those of Lipase 1 under different tested pHs and temperatures. In addition, Lipase 2 was more stable than Lipase 1 when treated with different metal ions, detergents, potential inhibitors, and organic solvents. In a combination of mutation and activity assays, catalytic triads of Ser, Asp, and His in Lipase 1 and Lipase 2 were demonstrated to be essential for maintaining enzyme activity. Phylogenetic analysis showed that both Lipase 1 and Lipase 2 belonged to lipase family III. Overall, our results indicate that deep-sea cold seep is a rich source for novel bacterial species that produce potentially unique cold-active enzymes. |
资助项目 | China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Major Research Plan of the National Natural Science Foundation[92051107] ; Key Deployment Projects of the Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; National Natural Science Foundation of China[31600035] |
WOS研究方向 | Microbiology |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000643311600001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/170606] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Sun, Chaomin; Wu, Shimei |
作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Guo, Chenchen,Zheng, Rikuan,Cai, Ruining,et al. Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium[J]. MICROORGANISMS,2021,9(4):16. |
APA | Guo, Chenchen,Zheng, Rikuan,Cai, Ruining,Sun, Chaomin,&Wu, Shimei.(2021).Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium.MICROORGANISMS,9(4),16. |
MLA | Guo, Chenchen,et al."Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium".MICROORGANISMS 9.4(2021):16. |
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