Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities

doi:10.1016/j.chemosphere.2020.129164

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities
	Yu, Chunlin 1,2; Yue, Yang 2; Yin, Xiujing 1,2; Li, Rongfeng 2,3; Yu, Huahua 2,3; Li, Pengcheng 2,3
刊名	CHEMOSPHERE
	2021-03-01
卷号	266 页码:13
关键词	Jellyfish Nemopilema nomurai Metalloprotease Phospholipase A(2) Geographical variation
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2020.129164
通讯作者	Yu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng()
英文摘要	Venom geographical variation is common among venomous animals. This phenomenon presents problems in the development of clinical treatments and medicines against envenomation. The venomous giant jellyfish Nemopilema nomurai, Scyphozoan, is a blooming jellyfish species in the Yellow Sea and the East China Sea that causes numerous jellyfish sting cases every year. Metalloprotease and phospholipase A(2) (PLA(2)) are the main components in Nemopilema nomurai venom and may activate many toxicities, such as hemolysis, inflammation and lethality. Geographical variation in the content and activity of these enzymes may cause different symptoms and therapeutic problems. For the first time, we verified metalloprotease and PLA(2) geographical variation in Nemopilema nomurai venom by performing a comparative analysis of 31 venom samples by SDS-PAGE, analyzing protease zymography, enzymatic activity, and drawing contour maps. Band locations and intensities of SDS-PAGE and protease zymograms showed geographical differences. The enzymatic activities of both metalloprotease and PLA(2) showed a trend of geographic regularity. The distribution patterns of these activities are directly shown in contour maps. Metalloproteinase activity was lower near the coast. PLA(2)-like activity was lower in the Southern Yellow Sea. We surmised that metalloproteinase and PLA(2)-like activities might be related to venom ontogeny and species abundance respectively, and influenced by similar environmental factors. This study provides a theoretical basis for further ecological and medical studies of Nemopilema nomurai jellyfish venom. (C) 2020 Elsevier Ltd. All rights reserved.
资助项目	National Key R&D Program of China[2019YFC0312605] ; National Key R&D Program of China[2017YFE0111100-04] ; National Natural Science Foundation of China[41776163] ; National Natural Science Foundation of China[41876164]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000605756800106
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170075]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yu, Huahua; Li, Pengcheng
作者单位	1.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yu, Chunlin,Yue, Yang,Yin, Xiujing,et al. Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities[J]. CHEMOSPHERE,2021,266:13.
APA	Yu, Chunlin,Yue, Yang,Yin, Xiujing,Li, Rongfeng,Yu, Huahua,&Li, Pengcheng.(2021).Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities.CHEMOSPHERE,266,13.
MLA	Yu, Chunlin,et al."Identifying and revealing the geographical variation in Nemopilema nomurai venom metalloprotease and phospholipase A(2) activities".CHEMOSPHERE 266(2021):13.