A Fish Galectin-8 Possesses Direct Bactericidal Activity

doi:10.3390/ijms22010376

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A Fish Galectin-8 Possesses Direct Bactericidal Activity
	Zhang, Tengfei 1,2,3; Jiang, Shuai 1,2,3; Sun, Li 1,2,3
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2021
卷号	22 期号:1 页码:15
关键词	galectin-8 Cynoglossus semilaevis bacterial binding bactericidal immune defense
DOI	10.3390/ijms22010376
通讯作者	Jiang, Shuai(sjiang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
英文摘要	Galectins are a family of animal lectins with high affinity for beta-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a tandem repeat galectin-8 from tongue sole Cynoglossus semilaevis (designated CsGal-8). CsGal-8 possesses conserved carbohydrate recognition domains (CRDs), as well as the conserved HXNPR and WGXEE motifs that are critical for carbohydrate binding. CsGal-8 was constitutively expressed in nine tissues of tongue sole and up-regulated in kidney, spleen, and blood by bacterial challenge. When expressed in HeLa cells, CsGal-8 protein was detected both in the cytoplasm and in the micro-vesicles secreted from the cells. Recombinant CsGal-8 (rCsGal-8) bound to lactose and other carbohydrates in a dose dependent manner. rCsGal-8 bound to a wide range of gram-positive and gram-negative bacteria and was co-localized with the bound bacteria in animal cells. Lactose, fructose, galactose, and trehalose effectively blocked the interactions between rCsGal-8 and different bacteria. Furthermore, rCsGal-8 exerted potent bactericidal activity against some gram-negative bacterial pathogens by directly damaging the membrane and structure of the pathogens. Taken together, these results indicate that CsGal-8 likely plays an important role in the immune defense against some bacterial pathogens by direct bacterial interaction and killing.
资助项目	National Key Research and Development Program of China[2018YFD0900500] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000606289800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169974]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Jiang, Shuai; Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Tengfei,Jiang, Shuai,Sun, Li. A Fish Galectin-8 Possesses Direct Bactericidal Activity[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(1):15.
APA	Zhang, Tengfei,Jiang, Shuai,&Sun, Li.(2021).A Fish Galectin-8 Possesses Direct Bactericidal Activity.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(1),15.
MLA	Zhang, Tengfei,et al."A Fish Galectin-8 Possesses Direct Bactericidal Activity".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.1(2021):15.