Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei

doi:10.1016/j.fsi.2020.07.014

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei
	Lv, Xinjia 1,3,5; Li, Shihao 2,3,5; Yu, Yang 2,3,5; Zhang, Xiaojun 2,3,5; Li, Fuhua 2,3,4,5
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-10-01
卷号	105 页码:393-404
关键词	Antimicrobial peptides Crustin RNA interference Gill microbiota Litopenaeus vannamei
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.07.014
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	Gills as the respiratory tissue of crustacean encounter various threats in the environment. The immune defense in gills is essential to the health of shrimp. In the present study, we identified a novel gill-abundant crustin, LvCrustin II-1, from the shrimp Litopenaeus vannamei. The full-length open reading frame of LvCrustin II-1 is 522 bp, which encodes 173 amino acid residues. The amino acid sequence of LvCrustin II-1 contained a signal peptide, a glycine rich region, a cysteine rich region and a whey acidic protein (WAP) domain. The expression level of LvCrustin II-1 was significantly up-regulated at different time points after Vibrio parahaemolyticus immersion. When LvCrustin II-1 was silenced by dsRNA interference, the count of bacteria colonies increased significantly in the hepatopancreas of shrimp after V. parahaemolyticus immersion, which indicated that the infection progress of pathogenic bacteria was accelerated after LvCrustin II-1 knockdown. Compared with the microbiota of seawater, the lower proportion of aquatic bacteria and higher proportion of symbiont in the gills microbiota of shrimp indicated the bacterial colonization was modulated by the host. Knockdown of LvCrustin II-1 changed the proportion of some potential pathogens and aquatic bacteria, which supported the idea that the new identified crustin in the gills played important roles in modulation of the microbiota community in shrimp. The present data provided new insights into the multiple functions of crustin in the immunity of shrimp.
资助项目	National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agricultural Research System[CARS-48]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000571746900005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168810]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xinjia,Li, Shihao,Yu, Yang,et al. Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:393-404.
APA	Lv, Xinjia,Li, Shihao,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2020).Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,105,393-404.
MLA	Lv, Xinjia,et al."Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 105(2020):393-404.