Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp,Litopenaeus vannamei

doi:10.3390/ijms21155444

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp,Litopenaeus vannamei
	Zhang, Xiaoxi 1,2,3,4; Yuan, Jianbo 1,3,4; Zhang, Xiaojun 1,3,4; Xiang, Jianhai 1,3,4; Li, Fuhua 1,3,4
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2020-08-01
卷号	21 期号:15 页码:15
关键词	juvenile hormone esterase-like carboxylesterase development molting penaeid shrimp
DOI	10.3390/ijms21155444
通讯作者	Yuan, Jianbo(yuanjb@qdio.ac.cn) ; Zhang, Xiaojun(xjzhang@qdio.ac.cn)
英文摘要	The sesquiterpenoid methyl farnesoate (MF), a juvenile hormone (JH) analog, plays important roles in many physiological processes of crustaceans, such as morphogenesis, molting and reproduction. Juvenile hormone esterase-like (JHE-like) carboxylesterase (CXE) is a key enzyme in MF degradation, playing a significant role in regulating MF titer. However, its function is barely known in shrimp. In this study, a total of 21 JHE-like CXEs (LvCXEs) were characterized in Pacific white shrimpLitopenaeus vannamei, based on the full genome and multi-transcriptomic data.LvCXEhas a conserved triplet catalytic site (Ser-Glu-His) and a characteristic GxSxG motif. MostLvCXEs were highly expressed in the hepatopancreas, which was the main site for MF degradation.LvCXEs containing a GESAG motif showed a specific expansion in theL. vannameigenome. Those GESAG-containingLvCXEs presented differential expressions at different larvae stages and different molting stages ofL. vannamei, which suggested their potential functions in development and molting. Additionally, when the transcription level of CXEs was inhibited, it could lead to failed molt and death ofL. vannamei. When we further detected the expression levels of the key ecdysone responsive transcription factors includingLvE75,LvBr-C,LvHr3andLvFtz-f1after the CXE inhibitor was injected intoL. vannamei, they all showed apparent down-regulation. These results suggested that the expansion ofLvCXEs in theL. vannameigenome should contribute to the regulation of metamorphosis at larvae stages and frequent molting during the growth ofL. vannamei.
资助项目	National Key R&D Program of China[2018YFD0900103] ; National Natural Sciences Foundation of China[31672632] ; National Natural Sciences Foundation of China[31972782] ; National Natural Sciences Foundation of China[41876167] ; China Agriculture Research System-48 (CARS-48)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000567073000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168755]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yuan, Jianbo; Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaoxi,Yuan, Jianbo,Zhang, Xiaojun,et al. Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp,Litopenaeus vannamei[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(15):15.
APA	Zhang, Xiaoxi,Yuan, Jianbo,Zhang, Xiaojun,Xiang, Jianhai,&Li, Fuhua.(2020).Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp,Litopenaeus vannamei.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(15),15.
MLA	Zhang, Xiaoxi,et al."Genomic Characterization and Expression of Juvenile Hormone Esterase-Like Carboxylesterase Genes in Pacific White Shrimp,Litopenaeus vannamei".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.15(2020):15.