IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus

doi:10.1016/j.fsi.2020.05.073

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus
	Yu, Chao 1,2,3; Zhang, Peng 1,2,3; Zhang, Teng-fei 1,2,3; Sun, Li 1,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-09-01
卷号	104 页码:228-236
关键词	Paralichthys olivaceus Interleukin 34 Inflammatory response Peripheral blood leukocytes Immune defense
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.05.073
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	Interleukin (IL)-34 is a relatively recently discovered cytokine with pleiotropic effects on various cellular activities, including immune response. In fish, the knowledge on the function of IL-34 is limited. In the present work, we investigated the function of Japanese flounder Paralichthys olivaceus IL-34 (PoIL-34) in association with inflammation and immune defense. PoIL-34 possesses the conserved structure of IL-34 superfamily and shares 21.52% sequence identity with murine IL-34. PoIL-34 expression was detected in a wide range of tissues of flounder, in particular intestine, and was regulated to a significant extent by bacterial infection in a time-dependent fashion. In vitro studies showed that recombinant PoIL-34 (rPoIL-34) bound peripheral blood leukocytes (PBLs) and promoted ROS production, acid phosphatase activity, and cellular resistance against bacterial infection. At the molecular level, rPoIL-34 enhanced the expressions of inflammatory cytokines and specific JAK and STAT genes. Similar stimulatory effects of rPoIL-34 were observed in vivo. When PoIL-34 was overexpressed in flounder, the expressions of pro- and anti-inflammatory mediators were significantly affected in a tissue-dependent manner, which correlated with an augmented ability of the fish to eliminate invading pathogens from tissues. Together, these results indicated that PoIL-34 regulated inflammatory response probably via specific JAK/STAT pathways and had a significant influence on the immune defense of flounder against bacterial infection.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key Research & Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000564750000013
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168537]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yu, Chao,Zhang, Peng,Zhang, Teng-fei,et al. IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:228-236.
APA	Yu, Chao,Zhang, Peng,Zhang, Teng-fei,&Sun, Li.(2020).IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus.FISH & SHELLFISH IMMUNOLOGY,104,228-236.
MLA	Yu, Chao,et al."IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus".FISH & SHELLFISH IMMUNOLOGY 104(2020):228-236.