Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection

doi:10.1016/j.fsi.2020.06.016

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection
	Sun, Yan-Ling 2,3; Guan, Xiao-Lu 2,3; Zhang, Peng 1,2,3; Li, Mo-Fei 2,3; Zhang, Jian 2,3; Sun, Li 2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-09-01
卷号	104 页码:439-446
关键词	Paralichthys olivaceus miRNA Ubiquitin-specific protease Pathogen Infection Immunity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.06.016
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	In this study, we examined the function of a Japanese flounder (Paralichthys olivaceus) microRNA (miRNA), polmiR-363-3p. We found that pol-miR-363-3p targets an ubiquitin-specific protease (USP), USP32. USP is a family of deubiquitinating enzymes essential to the functioning of the ubiquitin proteasome system. In mammals, USP32 is known to be associated with cancer and immunity. In fish, the function of USP32 is unknown. We found that flounder USP32 (PoUSP32) expression was detected in the major tissues of flounder, particularly intestine. In vitro and in vivo studies showed that pol-miR-363-3p directly regulated PoUSP32 in a negative manner by interaction with the 3 ' UTR of PoUSP32. Overexpression of pol-miR-363-3p or interference with PoUSP32 expression in flounder cells significantly blocked Streptococcus iniae infection. Consistently, in vivo knockdown of pol-miR-363-3p or overexpression of PoUSP32 enhanced dissemination of S. iniae in flounder tissues, whereas in vivo knockdown of PoUSP32 inhibited S. iniae dissemination. In addition, pol-miR-363-3p knockdown also significantly promoted the tissue dissemination of the viral pathogen megalocytivirus, which, as well as S. iniae, regulated pol-miR-363-3p expression. Together these results revealed an important role of pol-miR-363-3p in flounder immune defense against bacterial and viral infection.
资助项目	National Natural Science Foundation of China[31730100] ; National Natural Science Foundation of China[31902408] ; Marine S& T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000564306100002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168516]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yan-Ling,Guan, Xiao-Lu,Zhang, Peng,et al. Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:439-446.
APA	Sun, Yan-Ling,Guan, Xiao-Lu,Zhang, Peng,Li, Mo-Fei,Zhang, Jian,&Sun, Li.(2020).Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection.FISH & SHELLFISH IMMUNOLOGY,104,439-446.
MLA	Sun, Yan-Ling,et al."Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection".FISH & SHELLFISH IMMUNOLOGY 104(2020):439-446.