Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics

doi:10.1186/s12861-020-00223-3

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics
	Yang, Weihong 1,2,3; Huan, Pin 1,2,3; Liu, Baozhong 1,2,3
刊名	BMC DEVELOPMENTAL BIOLOGY
	2020-08-19
卷号	20 期号:1 页码:10
关键词	Cell movement F-actin dynamics Mollusk Morphogenesis Shell field Shell-formation gene
ISSN号	1471-213X
DOI	10.1186/s12861-020-00223-3
通讯作者	Huan, Pin(huanpin@qdio.ac.cn)
英文摘要	Background The morphogenesis of the shell field is an essential step of molluscan shell formation, which exhibits both conserved features and interlineage variations. As one major gastropod lineage, the patellogastropods show different characters in its shell field morphogenesis compared to other gastropods (e.g., the pulmonate gastropodLymnaea stagnalis), likely related to its epibolic gastrulation. The investigation on the shell field morphogenesis of patellogastropods would be useful to reveal the lineage-specific characters in the process and explore the deep conservation among different molluscan lineages. Results We investigated the early shell field morphogenesis in the patellogastropodLottia goshimaiusing multiple techniques. Electron microscopy revealed distinct morphological characters for the central and peripheral cells of the characteristic rosette-like shell field. Gene expression analysis and F-actin staining suggested that the shell field morphogenesis in this species predominantly relied on cell movement and F-actin dynamics, while BrdU assay revealed that cell proliferation contributed little to the process. We found constant contacts between ectodermal and meso/endodermal tissues during the early stages of shell field morphogenesis, which did not support the induction of shell field by endodermal tissues in general, but a potential stage-specific induction was indicated. Conclusions Our results emphasize the roles of cell movement and F-actin dynamics during the morphogenesis of the shell field inLo. goshimai, and suggest potential regulators such as diffusible factors and F-actin modulators. These findings reflect the differences in shell field morphogenesis of different gastropods, and add to the knowledge of molluscan larval shell formation.
资助项目	National Natural Science Foundation of China[41776157] ; National Key R&D Program of China[2018YFD0900104] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-1]
WOS研究方向	Developmental Biology
语种	英语
出版者	BMC
WOS记录号	WOS:000565127900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168447]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Huan, Pin
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yang, Weihong,Huan, Pin,Liu, Baozhong. Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics[J]. BMC DEVELOPMENTAL BIOLOGY,2020,20(1):10.
APA	Yang, Weihong,Huan, Pin,&Liu, Baozhong.(2020).Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics.BMC DEVELOPMENTAL BIOLOGY,20(1),10.
MLA	Yang, Weihong,et al."Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics".BMC DEVELOPMENTAL BIOLOGY 20.1(2020):10.