Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)

doi:10.3389/fcell.2020.00751

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)
	Wang, Lijuan 1,3; Wu, Zhihao 1,3; Zou, Congcong 1,2,3; Liang, Shaoshuai 1,3; Zou, Yuxia 1,3; Liu, Yan 1,3; You, Feng 1,3
刊名	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
	2020-08-05
卷号	8 页码:21
关键词	RNA editing N6-methyladenosine ovary testis Paralichthys olivaceus
ISSN号	2296-634X
DOI	10.3389/fcell.2020.00751
英文摘要	Adenosine-to-inosine (A-to-I) editing andN6-methyladenosine (m6A) are two of the most abundant RNA modifications. Here, we examined the characteristics of the RNA editing and transcriptome-wide m6A modification profile in the gonads of the olive flounder,Paralichthys olivaceus, an important maricultured fish in Asia. The gonadal differentiation and development of the flounder are controlled by genetic as well as environmental factors, and the epigenetic mechanism may play an important role. In total, 742 RNA editing events were identified, 459 of which caused A to I conversion. Most A-to-I sites were located in 3 ' UTRs, while 61 were detected in coding regions (CDs). The number of editing sites in the testis was higher than that in the ovary. Transcriptome-wide analyses showed that more than one-half of the transcribed genes presented an m6A modification in the flounder gonads, and approximately 60% of the differentially expressed genes (DEGs) between the testis and ovary appeared to be negatively correlated with m6A methylation enrichment. Further analyses revealed that the mRNA expression of some sex-related genes (e.g.,dmrt1andamh) in the gonads may be regulated by changes in mRNA m6A enrichment. Functional enrichment analysis indicated that the RNA editing and m6A modifications were enriched in several canonical pathways (e.g., Wnt and MAPK signaling pathways) in fish gonads and in some pathways whose roles have not been investigated in relation to fish sex differentiation and gonadal development (e.g., PPAR and RNA degradation pathways). There were 125 genes that were modified by both A-to-I editing and m6A, but the two types of modifications mostly occurred at different sites. Our results suggested that the presence of sex-specific RNA modifications may be involved in the regulation of gonadal development and gametogenesis.
资助项目	National Infrastructure of Fishery Germplasm Resources[2017DKA30470] ; National Natural Science Foundation of China[31772834] ; National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31702337] ; Youth Research Fund of Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology (Qingdao)[YQ2018NO01] ; Key R&D (public welfare) program of Shandong Province[2019GHY112005]
WOS研究方向	Cell Biology ; Developmental Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000563260500001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/168342]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lijuan,Wu, Zhihao,Zou, Congcong,et al. Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2020,8:21.
APA	Wang, Lijuan.,Wu, Zhihao.,Zou, Congcong.,Liang, Shaoshuai.,Zou, Yuxia.,...&You, Feng.(2020).Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus).FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,8,21.
MLA	Wang, Lijuan,et al."Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 8(2020):21.