CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)

doi:10.3389/fimmu.2020.01247

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	CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)
	Tang, Xueying 5,6; Huang, Baoyu 3,4,6; Lin, Siheng 4,6; Wang, Wei 2,3,4,6; Zhang, Guofan 2,3,4,6; Li, Li 1,2,3,6
刊名	FRONTIERS IN IMMUNOLOGY
	2020-07-14
卷号	11 页码:12
关键词	innate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88
ISSN号	1664-3224
DOI	10.3389/fimmu.2020.01247
通讯作者	Li, Li(lili@qdio.ac.cn)
英文摘要	Ostreid herpesvirus-1 microvariant (OsHV-1 mu Var) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated withCrassostrea gigasToll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 mu Var infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-kappa B reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 mu Var infections.
资助项目	National Key R&D Program of China[2018YFD0900304] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Key Deployment Project of Center for Ocean Mega-Research of Sciences[COMS2019Q06] ; Earmarked Fund for China Agriculture Research System[CARS-49] ; Scientific Research Staring Foundation of Minnan Normal University[4206-L21829]
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000556583000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168129]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Li
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Minnan Normal Univ, Dept Biol Sci & Biotechnol, Zhangzhou, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Tang, Xueying,Huang, Baoyu,Lin, Siheng,et al. CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)[J]. FRONTIERS IN IMMUNOLOGY,2020,11:12.
APA	Tang, Xueying,Huang, Baoyu,Lin, Siheng,Wang, Wei,Zhang, Guofan,&Li, Li.(2020).CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas).FRONTIERS IN IMMUNOLOGY,11,12.
MLA	Tang, Xueying,et al."CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)".FRONTIERS IN IMMUNOLOGY 11(2020):12.