Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus

doi:10.1016/j.gene.2020.144906

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus
	Wang, Wenxiang 1,2,3; Liang, Shaoshuai 1,2; Zou, Yuxia 1,2; Wu, Zhihao 1,2; Wang, Lijuan 1,2; Liu, Yan 1,2; You, Feng 1,2
刊名	GENE
	2020-09-10
卷号	755 页码:9
关键词	Olive flounder Paralichthys olivaceus Amh Expression Testicular differentiation and development Somatic cell
ISSN号	0378-1119
DOI	10.1016/j.gene.2020.144906
英文摘要	The olive flounder Paralichthys olivaceus, an important marine fish, shows gender differences in growth. The mechanism on its gonadal differentiation direction affected with exogenous factors still needs to be clarified. The anti-Mullerian hormone (amh) gene is involved in fish testicular differentiation and maintenance. The aim of this study was to investigate the expression of the flounder amh in tissues and the gonads. The quantitative expression analysis results showed that it was highly expressed in the testis, especially in the testis at stages I - IV (P < 0.05). Also, amh was detected in Sertoli cells surrounding spermatogonia and peripheral seminiferous lobule of the testis with in situ hybridization (ISH) and immunohistochemistry (IHC). During the differentiation period, the amh expression in the testis of the tamoxifen treatment group (100 ppm) was higher than that in the ovary of the 17 beta-estradiol (E2, 5 ppm) group, and the expression levels of amh during process of the male differentiation in the tamoxifen group were higher than those in the 17 alpha-methyltestosterone (MT, 5 ppm) group (P < 0.05). ISH results also exhibited that amh was expressed in the somatic cells that surrounded the germ cells of juvenile flounder similar to adult ones. Furthermore, the flounder gonads in the tamoxifen group maintained more germ cells and somatic cells than those in the MT group from 20 to 80 mm total length (TL). Especially, at 60 and 80 mm TL, the numbers of germ and somatic cells in the tamoxifen group were significantly higher than those in the MT group (P < 0.05). In summary, amh might initiate the process of testicular differentiation, and is involved in the early development and maintenance of testis.
资助项目	National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Key R&D Program of China[2018YFD0900202] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000555786400011
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/168108]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,et al. Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus[J]. GENE,2020,755:9.
APA	Wang, Wenxiang.,Liang, Shaoshuai.,Zou, Yuxia.,Wu, Zhihao.,Wang, Lijuan.,...&You, Feng.(2020).Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus.GENE,755,9.
MLA	Wang, Wenxiang,et al."Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus".GENE 755(2020):9.