Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273

doi:10.1111/jam.14764

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273
	Wu, Z.1; Zheng, R.2; Zhang, J.2; Wu, S.1
刊名	JOURNAL OF APPLIED MICROBIOLOGY
	2020-07-16
页码	13
关键词	biofilm polysaccharide Pseudomonas aeruginosa transcriptomic virulence factor
ISSN号	1364-5072
DOI	10.1111/jam.14764
英文摘要	Aims Relatively, few anti-biofilm polysaccharides againstPseudomonas aeruginosawere done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection ofP. aeruginosa. This study aims to investigate its anti-biofilm and anti-infection mechanism on transcriptional level. Methods and Results Herein, we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed afterP. aeruginosaPAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as two-component system PhoP-PhoQ and quorum sensing (QS) system LasI/LasR and RhlI/RhlR. Furthermore, when genesphoPandphoQwere disrupted, respectively, the reduction of biofilm formation and cell motility in mutant ophoPor ophoQwas also detected. Conclusion EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/LasR and RhlI/RhlR ofP. aeruginosa, which further regulated expression of genes involved in biofilm formation. Significance and Impact of the Study Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level.
资助项目	National Natural Science Foundation of China[31600035]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000548698500001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/167942]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wu, S.
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wu, Z.,Zheng, R.,Zhang, J.,et al. Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273[J]. JOURNAL OF APPLIED MICROBIOLOGY,2020:13.
APA	Wu, Z.,Zheng, R.,Zhang, J.,&Wu, S..(2020).Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273.JOURNAL OF APPLIED MICROBIOLOGY,13.
MLA	Wu, Z.,et al."Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273".JOURNAL OF APPLIED MICROBIOLOGY (2020):13.