Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells

doi:10.1007/s00343-019-9129-2

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells
	Zhou Shengnan 2; Liu Ge 1,3; Wu Shimei 2
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-05-01
卷号	38 期号:3 页码:826-833
关键词	Bacillus surfactin anticancer activity reactive oxygen species (ROS)
ISSN号	2096-5508
DOI	10.1007/s00343-019-9129-2
通讯作者	Wu Shimei(shimeiwu2016@126.com)
英文摘要	Marine bacterial strain Bacillus sp. CS30 exhibited high anticancer activity against Huh7.5 human liver cancer. We purified the corresponding anticancer agent by sequential acidic precipitation, methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), then analyzed it in mass spectrometry. Based on the results of purification and mass spectrometry, we deduced that the anticancer agent was the same component as our previously purified antifungal agent surfactin CS30-2. However, to the best of our knowledge, this is the first report on the surfactin possessing both antifungal and anticancer activities. Surfactin CS30-2 was demonstrated to exhibit high anticancer activity in a dose-dependent manner against Huh7.5 liver cancer cells. Further investigation showed that surfactin CS30-2 induced the increased generation of reactive oxygen species (ROS) and severe disruption of cell membrane, thus leading to cell death. However, unlike previously reported surfactins, surfactin CS30-2 caused cancer cell death via necrosis instead of apoptosis.
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000536606000020
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167557]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wu Shimei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhou Shengnan,Liu Ge,Wu Shimei. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(3):826-833.
APA	Zhou Shengnan,Liu Ge,&Wu Shimei.(2020).Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(3),826-833.
MLA	Zhou Shengnan,et al."Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.3(2020):826-833.