Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells

doi:10.1016/j.ijbiomac.2020.02.087

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells
	Wang, Jing 1,2,3,4; Zhang, Quanbin 2,3; Li, Shuang 2,3,4; Chen, Zhihang 2,3,4; Tan, Jiaojiao 2,3,4; Yao, Jianting 2,3; Duan, Delin 1,2,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2020-05-01
卷号	150 页码:304-314
关键词	Fucoidan ECM-receptor interaction Fibronectin
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2020.02.087
通讯作者	Duan, Delin(dlduan@qdio.ac.cn)
英文摘要	Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD). Currently, approximately 20-40% of individuals with diabetes are diagnosed with DN. Mesangial cells (MCs) are critical for maintaining and regulating glomerular filtration, and the abnormal proliferation of MCs causes the accumulation of mesangial extracellular matrix (ECM), further promoting glomerular dysfunction and renal diseases. Low molecular weight fucoidan (LMWF) extracted from Saccharina japonica could alleviate DN, but the mechanism was not analysed. Based on the ability of LMWF to ameliorate the human renal mesangial cell (HRMC) injury caused by advanced glycation end products (AGEs), we identified fibronectin (FN) as the most obviously impacted protein in the ECM-receptor interaction by proteomic analysis. The co-localization of LMWF and FN indicated direct interaction between them, and surface plasmon resonance (SPR) analysis confirmed the specific binding with a K-D of 453.7 mu mol L-1. Positively charged protamine sulfate (PS) promoted the combination of LMWF and HRMCs and further enhanced the effect of LMWF on HRMC injury. Our results indicated that LMWF alleviates the HRMC injury caused by AGEs via binding FN and inhibiting the ECM-receptor interaction pathway. These results provide a foundation for the in-depth analysis of the mechanism of polysaccharide functions. (c) 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
资助项目	Marine Science and Technology Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; Bilateral Joint Research Project between China and Japan[2017YFE0130900]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000525869500031
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167163]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing,Zhang, Quanbin,Li, Shuang,et al. Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,150:304-314.
APA	Wang, Jing.,Zhang, Quanbin.,Li, Shuang.,Chen, Zhihang.,Tan, Jiaojiao.,...&Duan, Delin.(2020).Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,150,304-314.
MLA	Wang, Jing,et al."Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 150(2020):304-314.