PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles

doi:10.1186/s12870-019-2125-z

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles
	Liu, Shun 2,3,4; Hu, Zi-Min 2,3; Zhang, Quansheng 1; Yang, Xiaoqi 2,3,4; Critchley, Alan T.5; Duan, Delin 2,3
刊名	BMC PLANT BIOLOGY
	2019-11-27
卷号	19 期号:1 页码:14
关键词	Seaweed Dehydration Ubiquitination Phosphatidylinositol signaling system Weighted gene co-expression network analysis
ISSN号	1471-2229
DOI	10.1186/s12870-019-2125-z
通讯作者	Hu, Zi-Min(huzm@gdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
英文摘要	Background Intermittent dehydration caused by tidal changes is one of the most important abiotic factors that intertidal seaweeds must cope with in order to retain normal growth and reproduction. However, the underlying molecular mechanisms for the adaptation of red seaweeds to repeated dehydration-rehydration cycles remain poorly understood. Results We chose the red seaweed Gloiopeltis furcata as a model and simulated natural tidal changes with two consecutive dehydration-rehydration cycles occurring over 24 h in order to gain insight into key molecular pathways and regulation of genes which are associated with dehydration tolerance. Transcription sequencing assembled 32,681 uni-genes (GC content = 55.32%), of which 12,813 were annotated. Weighted gene co-expression network analysis (WGCNA) divided all transcripts into 20 modules, with Coral2 identified as the key module anchoring dehydration-induced genes. Pathways enriched analysis indicated that the ubiquitin-mediated proteolysis pathway (UPP) and phosphatidylinositol (PI) signaling system were crucial for a successful response in G. furcata. Network-establishing and quantitative reverse transcription PCR (qRT-PCR) suggested that genes encoding ubiquitin-protein ligase E3 (E3-1), SUMO-activating enzyme sub-unit 2 (SAE2), calmodulin (CaM) and inositol-1,3,4-trisphosphate 5/6-kinase (ITPK) were the hub genes which responded positively to two successive dehydration treatments. Network-based interactions with hub genes indicated that transcription factor (e.g. TFIID), RNA modification (e.g. DEAH) and osmotic adjustment (e.g. MIP, ABC1, Bam1) were related to these two pathways. Conclusions RNA sequencing-based evidence from G. furcata enriched the informational database for intertidal red seaweeds which face periodic dehydration stress during the low tide period. This provided insights into an increased understanding of how ubiquitin-mediated proteolysis and the phosphatidylinositol signaling system help seaweeds responding to dehydration-rehydration cycles.
资助项目	National Natural Science Foundation of China[41761144057] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1]
WOS研究方向	Plant Sciences
语种	英语
出版者	BMC
WOS记录号	WOS:000501294600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163783]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Hu, Zi-Min; Duan, Delin
作者单位	1.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Univ Cape Breton, Verschuren Ctr Sustainabil Energy & Environm, Sydney, NS, Canada
推荐引用方式 GB/T 7714	Liu, Shun,Hu, Zi-Min,Zhang, Quansheng,et al. PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles[J]. BMC PLANT BIOLOGY,2019,19(1):14.
APA	Liu, Shun,Hu, Zi-Min,Zhang, Quansheng,Yang, Xiaoqi,Critchley, Alan T.,&Duan, Delin.(2019).PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles.BMC PLANT BIOLOGY,19(1),14.
MLA	Liu, Shun,et al."PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles".BMC PLANT BIOLOGY 19.1(2019):14.