The Eastern Tianshan Orogenic Belt of the Central Asian Orogenic Belt and the Beishan terrane of the Tarim Block, NW China, host numerous Fe deposits. The Cihai Fe deposit (>90 Mt at 45.6 % Fe) in the Beishan terrane is diabase-hosted and consists of the Cihai, Cinan, and Cixi ore clusters. Ore minerals are dominantly magnetite, pyrite, and pyrrhotite, with minor chalcopyrite, galena, and sphalerite. Gangue minerals include pyroxene, garnet, hornblende and minor plagioclase, biotite, chlorite, epidotite, quartz, and calcite. Pyrite from the Cihai and Cixi ore clusters has similar Re–Os isotope compositions, with ∼14 to 62 ppb Re and ≤10ppt common Os. Pyrrhotite has ∼5 to 39 ppb Re and ∼0.6 ppb common Os. Pyrite has a mean Re–Os model age of 262.3±5.6 Ma (n=13), in agreement with the isochron regression of 187Os vs. 187Re. The Re–Os age (∼262 Ma) for the Cihai Fe deposit is within uncertainty in agreement with a previously reported Rb–Sr age (268±25 Ma) of the hosting diabase, indicating a genetic relationship between magmatism and mineralization. Magnetite from the Cihai deposit has Mg, Al, Ti, V, Cr, Co, Ni, Mn, Zn, Ga, and Sn more elevated than that of typical skarn deposits, but both Vand Ti contents lower than that of magmatic Fe–Ti–V deposits. Magnetite from these two ore clusters at Cihai has slightly different trace element concentrations. Magnetite from the Cihai ore cluster has relatively constant trace element compositions. Some magnetite grains from the Cixi ore cluster have higher V, Ti, and Cr than those from the Cihai ore cluster. The compositional variations of magnetite between the ore clusters are possibly due to different formation temperatures. Combined with regional tectonic evolution of the Beishan terrane, the Re–Os age of pyrite and the composition of magnetite indicate that the Cihai Fe deposit may have derived from magmatic– hydrothermal fluids related to mafic magmatism, probably in an extensional rift environment.