In this study, an electron microprobe analyzer (EMPA) was used to map the spatial distribution and the occurrence of invisible gold in pyrite from the Liulincha gold ore belt. EPMA data show that gold mainly occurs as submicroscopic-microscopic inclusions. From the contrast of the major guide elements of pyrite from the Liulincha gold ore belt and those from four hydrothermal-type gold deposits in the Jiaodong region, we can see the pyrites were formed in two stages: the pyrite from wall rock is mainly sedimentogenic, with simple structure; and the pyrite from ore body experienced early sedimentary process to late hydrothermal activity, the pyrite is regular in crystal form and exhibits fractured structure.