High Oxidation Stability of Tea Polyphenol-stabilized Highly Crosslinked UHMWPE Under an in Vitro Aggressive Oxidative Condition

doi:10.1097/CORR.0000000000000771

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	High Oxidation Stability of Tea Polyphenol-stabilized Highly Crosslinked UHMWPE Under an in Vitro Aggressive Oxidative Condition
	Ren, Y ; Wei, X ; Wei, ST ; Wang, FY ; Wang, J ; Xu, JZ ; Xu, L ; Gul, RM ; Li, ZM
刊名	CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
	2019
卷号	477 期号:8 页码:1947-1955
关键词	MOLECULAR-WEIGHT POLYETHYLENE FREE-RADICALS MECHANICAL-PROPERTIES NATURAL ANTIOXIDANTS ALPHA-TOCOPHEROL SYNOVIAL-FLUID TOTAL HIP STERILIZATION WEAR DEGRADATION
ISSN号	0009-921X
DOI	10.1097/CORR.0000000000000771
文献子类	期刊论文
英文摘要	Background Synovial fluid components, especially lipids, can trigger oxidation of ultrahigh-molecular-weight polyethylene (UHMWPE) artificial joint components in vivo. The use of antioxidants such as vitamin E effectively diminishes the oxidative cascade by capturing free radicals and reducing the oxidation potential of UHMWPE implants. Using a thermo-oxidative aging method, we recently found that tea polyphenols can enhance the oxidation resistance of irradiated UHMWPE in comparison with commercial vitamin E. However, it is yet unknown whether tea polyphenols can reduce lipid-induced oxidation. Questions/purposes We explored whether tea polyphenol-stabilized UHMWPE would exhibit (1) lower squalene absorption; (2) stronger oxidation resistance; and (3) lower content of free radicals than vitamin E-stabilized UHMWPE under a physiologically-motivated in vitro accelerated-aging model. Methods Tea polyphenol (lipid-soluble epigallocatechin gallate [lsEGCG]) and vitamin E were blended with UHMWPE powders followed by compression molding and electron beam irradiation at 100 and 150 kGy. Small cubes (n = 3, 60 mg, 4 x 4 x 4 mm(3)) cut from the blocks were doped in squalene at 60 degrees, 80 degrees, 100 degrees, and 120 degrees C for 2 hours. Gravimetric change of the cubes after squalene immersion was measured to assess absorption. Thin films (n = 3, similar to 60 mu m) were also microtomed from the blocks and were doped at 120 degrees C for 24 hours. Oxidation induction time (n = 3, 5 mg of material from the cubes) and incipient oxidation temperature (n = 3, thin films) were obtained to determine the oxidation stability. Signal intensity of the free radicals, obtained by electron spin resonance spectroscopy, was used to qualitatively rank the antioxidant ability of vitamin E and lsEGCG. Results Squalene absorption was comparable between lsEGCG/UHMWPE and vitamin E/UHMWPE at a given temperature and radiation dose. The oxidation induction time of 100 kGy-irradiated UHMWPE was increased with /sEGCG compared with vitamin E except at 120 degrees C. For example, the oxidation induction time value of 100 kGy-irradiated lsEGCG/UHMWPE immersed at 60 degrees C was 25.3 minutes (24.2-27.8 minutes), which was 8.3 minutes longer than that of 100 kGy-irradiated vitamin E/UHMWPE which was 17.0 minutes (15.0-17.1 minutes) (p = 0.040). After squalene immersion at 120 degrees C, the incipient oxidation temperature of 100 and 150 kGy irradiated lsEGCG/UHMWPE was 234 degrees C (227-240 degrees C) and 227 degrees C (225-229 degrees C), which was higher than vitamin E-stabilized counterparts with value of 217 degrees C (214-229 degrees C; p = 0.095) and 216 degrees C (207-218 degrees C; p = 0.040), respectively. The electron spin resonance signal of 150 kGy irradiated /sEGCG/UHMWPE was qualitatively weaker than that of 150 kGy irradiated vitamin E/UHMWPE. Conclusions lsEGCG-stabilized UHMWPE demonstrated higher oxidation resistance than vitamin E-stabilized UHMWPE after squalene immersion, likely because lsEGCG donates more protons to eliminate macroradicals than vitamin E.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32078]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, Chengdu 610065, Peoples R China; 2.Tongji Univ, Shanghai Peoples Hosp 10, Dept Stomatol, Shanghai, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China; 4.Univ Engn & Technol, Dept Mech Engn, Peshawar, Pakistan
推荐引用方式 GB/T 7714	Ren, Y,Wei, X,Wei, ST,et al. High Oxidation Stability of Tea Polyphenol-stabilized Highly Crosslinked UHMWPE Under an in Vitro Aggressive Oxidative Condition[J]. CLINICAL ORTHOPAEDICS AND RELATED RESEARCH,2019,477(8):1947-1955.
APA	Ren, Y.,Wei, X.,Wei, ST.,Wang, FY.,Wang, J.,...&Li, ZM.(2019).High Oxidation Stability of Tea Polyphenol-stabilized Highly Crosslinked UHMWPE Under an in Vitro Aggressive Oxidative Condition.CLINICAL ORTHOPAEDICS AND RELATED RESEARCH,477(8),1947-1955.
MLA	Ren, Y,et al."High Oxidation Stability of Tea Polyphenol-stabilized Highly Crosslinked UHMWPE Under an in Vitro Aggressive Oxidative Condition".CLINICAL ORTHOPAEDICS AND RELATED RESEARCH 477.8(2019):1947-1955.